- Research article
- Open Access
Bio-analytical chiral chromatography method for the enantioselective separation of carbinoxamine maleate in human plasma
Journal of Analytical Science and Technology volume 6, Article number: 31 (2015)
A selective chiral high-performance liquid chromatography (HPLC) method was developed and validated to separate and quantify the (d) and (l) carbinoxamine enantiomers in human plasma.
Plasma samples were extracted by liquid-liquid extraction. The separation of carbinoxamine enantiomers and internal standard (IS, pargeverine hydrochloride) was achieved on an amylose tris(5-chloro-2-methylphenylcarbamate) column with a mobile phase of n-Hexane/isopropanol/ethanol/diethyl amine (850:75:75:0.1, v/v/v/v) at a flow rate of 0.8 mL/min. The ultraviolet (UV) detection wavelength was set at 220 nm.
Baseline separation of carbinoxamine enantiomers and IS, free from endogenous interferences, was achieved in less than 15 min. Ratio of peak area of each enantiomer to IS was used for quantification of plasma samples. Linear calibration curves were obtained over the range of 20–7500 g/mL in plasma for both enantiomers (R 2 > 0.99). The mean extraction recoveries were 103.8 ± 1.5 and 94.5 ± 1.8 % for (d) and (l) enantiomers of carbinoxamine enantiomers and 96.35 % for IS from human plasma. The mean relative error (RE %) of accuracy and the mean relative standard deviation (RSD %) of intra-day and inter-day precision for both enantiomers were <10 %.
The method was validated with accuracy, precision, recovery, and stability and can be used to determine the pharmacokinetics of carbinoxamine enantiomers in human plasma.
In the pharmaceutical industry, enantiomeric separation has become an important area of study as the single-enantiomer drugs are found to be safer than racemic drugs (Pharma Book 2009). Carbinoxamine (CA) is an oral antihistamine agent that acts primarily by competing with the histamine in binding to H1 receptor. It is used as monotherapy or in combination with pseudoephedrine (or) paracetamol in the management of hay fever and allergic conjunctivitis. It is also has anticholinergic properties, and because of which, it is used in the treatment of Parkinson’s disease. The chemical structure of CA was shown in Fig. 1, with one chiral center attached with pyridyl ring and chlorphenyl ring. It is commercially available as the maleic acid salt (River’s Edge 2011). Investigations have shown that CA was used as the racemic mixture clinically.
Ethanolamine class of H1 antihistamines were proven to show stereoselectivity in binding to the receptors (Casy et al. 1992). Doxylamine which is a similar kind of molecule to that of carbinoxamine showed that its (d) or (+) form has more binding affinity compared to its antipode (Tadiboyina et al. 2015). It is currently unclear whether CA enantiomers also possess different pharmacodynamics, toxicological, or pharmacokinetic properties. To determine the pharmacokinetic properties of CA enantiomers in vivo, it is necessary to establish a reliable method for the detection of CA enantiomers in biological samples. Table 1 shows some methods for determination of racemic CA in biological fluids and dosage forms. So far, very few studies were carried out to separate carbinoxamine into its individual enantiomers. Yi-Fen et al. (2000) developed a quantitative chiral analysis of carbinoxamine, doxylamine, and orphenadrine by capillary zone electrophoresis, but the method is not validated and not applied for bio-analytical studies. Therefore, it is unknown whether this method provides sufficient sensitivity and selectivity for bio-analytical studies. To our knowledge, no enantioselective methods have been developed for the determination of CA enantiomers in biological samples using a polysaccharide-based chiral column. In this paper, we describe the development of a stereoselective method for CA in the presence of internal standard pargeverine hydrochloride (PGV) (Fig. 2) by the normal phase method. Stationary phase was selected on the basis of functional groups present in the compound; mobile phase composition was systemically studied and finally optimized the method conditions by using eco-friendly solvents.
Chemicals and materials
Carbinoxamine maleate and pargeverine hydrochloride standards were gifted by RL Fine Chemicals, Bengaluru, India. n-Hexane, 2-proponol, ethanol, and diethylamine were procured from Merck, Mumbai, India. High-performance liquid chromatography (HPLC)-grade water was obtained from a Milli-Q unit (Millipore, Milford, USA). Blank human plasma was obtained from JSS Medical College and Hospital, JSS University, Mysuru, India.
The instrumentation consisted of a Shimadzu Prominence LC-20 AD ultrafast liquid chromatography (UFLC) equipped with a 1260 binary pump VL (35 MPa), Prominence SIL-20ACHT Auto sampler, and Prominence SPD-M20A Diode array detector. All weighings for analysis were performed on a Shimadzu electronic analytical balance AY-220 (Shimadzu). Plasma samples were sonicated on a Mark ultrasonic sonicator and vertexed on a Remi cyclomixer; centrifugation was done using REMI centrifuge model number 412 LAG (REMI Instruments Division, Vasai, India). Water used for analysis was prepared from Millipak Express 20 filter unit. Microsoft Excel 2007 was used for analysis of validation results.
The chromatographic separations were carried out on amylose tris(5-chloro-2-methylphenylcarbamate) column. The mobile phase was a mixture of n-Hexane/isopropanolthanol/diethyl amine (850:75:75:0.1, v/v/v/v) with 0.8 mL/min of flow rate, and detection was performed by a photodiode array detector (PDA) at 220 nm.
Preparation of stock and standard solutions
Stock solutions of CA and PGV in isopropanol were prepared separately at a concentration of 10 μg/mL. A series of standard mixture solutions were prepared by the appropriate dilution of the stock standard solutions with isopropanol to a concentration range of 0.4–1000 μg/mL for CA and 40 μg/mL for PGV.
Preparation of samples
The plasma samples were thawed at room temperature. The samples were vortexed adequately before pipetting. To a 200-μL aliquot of plasma, 100 μL of racemic CA stock solution (0.4–1000 μg/mL), 100 μL of IS solution (40 μg/mL), and 100 μL of 1 M NaOH were added and vortexed for 1 min in a 2-mL Eppendorf tube. The mixed samples were then extracted with 1.5 mL of dichloromethane: n-Hexane (1:2), by vortex mixing for 2 min. After centrifugation at 5000 rpm for 15 min, 1.0 mL of the upper organic layer was transferred to another tube. Extracts were concentrated to dryness at the 40 °C under a gentle stream of nitrogen and reconstituted with 150 μL of ethanol. A 25-μL aliquot of the solution was injected into the UFLC system for the analysis.
Bio-analytical method validation
The plasma calibration curve was constructed using a blank sample (matrix sample processed without analyte or internal standard), a zero sample (matrix sample processed without analyte but with internal standard), and six non-zero samples (matrix samples processed with analyte and internal standard) covering the expected range including lower limit of quantification (LLOQ), 20–7500 ng/mL with 2000 ng/mL of IS concentration.
The efficiency of carbinoxamine enantiomers and IS extraction from human plasma was determined by comparing the responses of the analyte extracted from six reproduce QC samples with the response of analyte from neat standards at equivalent concentrations by a liquid-liquid extraction process. Recoveries of CA were determined at the LLOQ, QC low, QC medium, and QC high concentrations that is, 20, 300, 1200, and 5000 ng/mL. Whereas the recovery of the IS was determined at a single concentration of 2000 ng/mL.
Accuracy and precision
The intra-assay accuracy and precision were estimated by analyzing six replicates containing CA at four different QC levels, that is, 20, 300, 1200, and 5000 ng/mL. The inter-assay precision was estimated by studying the quality control samples on four different runs. The criteria for acceptability of accuracy data within 85–115 % of the actual values and ±15 % relative standard deviation (RSD) except for LLOQ for precision.
Stability tests were conducted to evaluate the stability of CA enantiomers in plasma samples under different conditions. In-injector stability (24 h), bench-top stability (12 h), freeze-thaw stability (3 cycles), and freezer stability (80 ± 10° C for 25 days) were tested at LQC (300 ng/mL) and HQC (5000 ng/mL) levels using six replicates at each level. Samples were said to be stable if assay values were within the acceptable limits (i.e., 85–115 % accuracy and ±15 % RSD from fresh samples).
Results and discussion
Method development and optimization
Selection of chiral stationary phase
The finest chiral stationary phases (CSP) for the separation of aromatic compounds with functional groups such as carbonyl, alcohol, and amine (like carbinoxamine) are amylose-based polysaccharide stationary phases (LCGC 2008). Immobilized polysaccharide columns are compatible with a wide range of solvents; hence, they can be operated in both reverse phase and normal phase which increased the range of applications (Othman et al. 2012). Hence, the coated amylose-based polysaccharide column was selected for screening.
The CSP is amylose tris(5-chloro-2-methylphenylcarbamate) bonded to silica gel. The separation of enantiomers may be attributed to hydrogen bonding interactions between the amine group of solute and the hydrophilic carbamate group on the CSP. Steadying effect on the solute-CSP complex will be there for the solutes having aromatic functional groups (Irving and Rose 1987). This type of steadying effect (or) stabilization effect may also exist in CA owing to the presence of aromaticity.
Selection and optimization of additives
As polysaccharide-based CSPs generally show a higher success rate in resolving enantiomers under normal-phase elution (Brian 2010); hence, this mode was explored first. The selection of mobile phases was initiated with traditional alkane/alcohol mixtures (Chiral Technologies 2004). n-Hexane was given first preference as it is comparatively green (Paul et al. 2012). For separation of most of the analytes containing basic and/or acidic functional groups, the additive plays a major role in increasing the chromatographic efficiency (Zhang et al. 2012). The additives were used in the present word in order to improve the peak shape. All chromatographic parameters were kept constant (flow rate, 0.8 mL/min, ambient column oven temperature; injection volume, 25 μL; wavelength, 220 nm) except the % additive added. Diethylamine and triethylamine were often used as a modifier for basic drugs with amine groups to ensure elution from the column and to obtain good peak shape (Toussaint et al. 2000). Hence 0.01 % diethylamine was introduced into the mobile phase, CA enantiomers were eluted at around 10 min, and the peaks were highly asymmetric. Further increase in diethylamine content (0.1 %) has significant impact on the peak shape, whereas the mobile phase without diethylamine significantly distorted the peak shape.
Selection of alcoholic modifiers
In chiral separations, the alcohols play a major role in creating a selectivity difference (Tang 1996). Hence, the ratio of the mobile phase components n-Hexane/alcohol/diethylamine was fixed at 85:15:0.01 (v/v/v) and the effect of different alcohols on selectivity was studied (Fig. 3). The details of different alcohols screened (trials 1–5) and their results are given in Table 2. The results of trials 3 and 4 appeared to be complementary. Ethanol alone as an alcoholic component in trial 2 produces broad peaks with slow elution. Isopropanol alone eluted the enantiomers very early, and because of which, the resolution is not proper. Hence, to achieve the complementary effects of trials 2 and 3, ethanol and isopropanol was tested together (trial 5). Since trial 5 showed a significant improvement in the retention and resolution, an equal ratio of ethanol and isopropanol were used for the analysis. Hence, at the optimized chromatographic conditions, the internal standard and the enantiomers were eluted at 4.9, 5.8, and 6.79 min, respectively, passing all the chromatographic parameters (Table 3).
Optical rotation of CA enantiomers
The order of elution of (‘d’) or (+) and (‘l’) or (−) CA enantiomers was determined by collecting eluent fractions from repeated injections (Dalia et al. 2008). There were ~45 injections made into the UFLC, with 150 μg racemate per injection. Figure 4 depicts the spectral comparison of both the enantiomers, which shows that both the peaks represent the same drug. After compiling the eluent fractions corresponding to each enantiomer, they were dried by passing the nitrogen gas and reconstituted in methanol and the optical rotation was determined using a Jasco P-2000 (JASCO Analytical instruments, USA).
The bio-analytical validation was performed according to FDA guidelines (FDA guideline 2001).
The calibration standard curve had a reliable reproducibility over the standard concentrations across the calibration range. The calibration curve was constructed by determining the best fit of peak area ratios (peak area analyte/peak area IS) vs. concentration and fitted to the y = mx + c. The average regression (n = 3) was found to be ≥0.996 for both the (d) and (l) enantiomers of CA. The lowest concentration with the RSD <20 % was taken as the LLOQ and was found to be 20 ng/mL.
One-step liquid-liquid extraction technique gave adequate recovery and cleaner samples (Fig. 5). The results of the comparison of neat standards vs. human plasma-extracted standards were estimated for CA enantiomers at low (300 ng/mL), medium (1200 ng/mL), and high (5000 ng/mL) levels, and the mean recovery for (d) and (l) enantiomers of CA was shown in Table 4. The recovery of internal standard (2000 ng/mL) was 96.35 %.
Accuracy and precision
The results of accuracy and precision throughout the standard curve for (d) and (l) enantiomers of CA are presented in Table 5. The RSD values of intra-day and inter-day precision for two enantiomers were less than 4.2 %. All the values of accuracy and precision including the LLOQ fell within the limits which were considered as acceptable.
The concentrations of CA at low (300 ng/mL) and high (5000 ng/mL) level samples deviated within ±15 % of the nominal concentrations in stability tests like in-injector (24 h), bench-top (12 h), three repeated freeze-thaw cycles, and freezer stability (−80 ± 10 °C for 25 days) (Table 6). The results were found to be within the assay variability limits during the entire process.
The developed method was found to be sensitive, specific, and robust for quantification of both the (d) and (l) enantiomers of carbinoxamine in human plasma. The method involved sample preparation with adequate recovery by liquid-liquid extraction. Method parameters like % alcoholic modifier, % basic additive, and flow rate were successfully adjusted. The method ensured as a robust one from the responses obtained. Even for injection volume 25 μL the method is quite sensitive. The method is user-friendly because of using green solvents n-Hexane and ethanol. It is concluded that the method is suitable for the routine quantification of (d) and (l) enantiomers of CA in human plasma.
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Authors are thankful to The Principal, JSS College of Pharmacy, JSS University, Mysuru, India for providing the necessary facilities. The authors express gratitude to Dr. Ramesh A. R, Vice President, R L Fine Chem, Bengaluru, for providing the racemic carbinoxamine maleate drug as a gift sample. Authors are beholden to University Grants Commission, New Delhi for the financial assistance (Ref: F.No.40-271/2011 (SR) dated 10.01.2013).
The authors declare that they have no competing interests.
ST has carried out the major research work presented in this article. BMG, acted as guide for research work and helped in giving the inputs for various topics carried out in this research work. BKI, helped in preparing and drafting the manuscript
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Tadiboyina, S., Gurupadayya, B.M. & Inturi, B.K. Bio-analytical chiral chromatography method for the enantioselective separation of carbinoxamine maleate in human plasma. J Anal Sci Technol 6, 31 (2015) doi:10.1186/s40543-015-0072-3
- Chiral chromatography
- Carbinoxamine enantiomers and human plasma