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A facile synthesis of GO/CuO-blended nanofiber sensor electrode for efficient enzyme-free amperometric determination of glucose


The development of biosensors with innovative nanomaterials is crucial to enhance the sensing performance of as-prepared biosensors. In the present research work, we prepared copper (II) oxide (CuO) and graphene oxide (GO) composite nanofibers using the hydrothermal synthesis route. The structural and morphological properties of as-prepared GO/CuO nanofibers were analyzed using an X-ray diffractometer, field-emission scanning, energy dispersive X-ray analysis, Fourier transmission infrared spectroscopy, Raman spectroscopy, and X-ray photoelectron spectroscopy. The results indicated GO/CuO nanofibers exhibit nanosized diameters and lengths in the order of micrometers. These GO/CuO nanofibers were employed to prepare non-enzymatic biosensors (GO/CuO nanofibers/FTO (fluorine-doped tin oxide)) modified electrodes for enhanced glucose detection. The sensing performance of the biosensors was evaluated using linear sweep voltammetry (LSV) and chronoamperometry in phosphate buffer solution (PBS). GO/CuO/FTO biosensor achieved high sensitivity of 1274.8 μA mM−1cm−2 having a linear detection range from 0.1 to 10 mM with the lower detection limit (0.13 μM). Further, the prepared biosensor showed good reproducibility repeatability, excellent selectivity, and long-time stability. Moreover, the technique used for the preparation of the GO/CuO composite is simple, rapid, cost-effective, and eco-friendly. These electrodes are employed for the detection of glucose in blood serum with RSD ~ 1.58%.


Diabetes is a widespread disease affecting millions of populations and predicted to be the major cause of death as it can damage neural systems in humans. It is essential for diabetic patients to frequently monitor and maintain glucose levels. Hence, an effective diagnosis of diabetes and a reliable glucose monitoring system are necessary. Extensive majors have been taken to control diabetes through several monitoring systems. Glucose biosensors have a great contribution in monitoring glucose levels of diabetic patients (Makaram et al., 2014; Bruen et al., 2017; Gopalan et al. 2017; Gopalan et al. 2016; Sai-Anand et al. 2014). Enzymatic electrochemical sensors suggest good selectivity and sensitivity but due to complex mobility, lack of stability and reproducibility limit their performance (Gopalan et al. 2013, Haldorai et al. 2016). These limitations are overcome by the development of enzyme-free glucose sensor devices through direct oxidization of glucose in the form oxidized layer (Sai-Anand et al. 2019). Glucose oxidase is usually practiced as an enzyme in most of the glucose biosensors. Amperometry is another widely used technique for glucose detection (Peter and Heineman, 1996, Thévenot et al., 2001). Glucose oxidase performs a major role in the oxidation of β-d-glucose to d-glucono-δ-lactone. Hydrogen peroxide (H2O2) is formed as a byproduct in a catalytic reaction and oxygen as an electron acceptor. A simple technique, less analysis time, cost effective, and low detection capability are some advantages of using amperometry (Thunkhamrak et al., 2020). Regardless of excellent sensitivity and low detection limit in few nanocomposites, an enzymatic glucose sensor limits the application of sensors due to high production cost (G. Gnana kumar et al., 2017). Nowadays, graphene has attracted researchers due to the larger specific superficial area, purity, high conductivity, and low cost (Liu et al., 2019). It has diversified applications in various areas such as batteries (Ji et al., 2016), biomedical (Mallick et al., 2018), supercapacitors (Hota et al. 2020), printable graphene electronics (Hu Etal. 2016) gas sensors (Nakate et al. 2020), and biosensors (Bai et al. 2020). In chemical synthesis, graphene oxide (GO) is reduced to eliminate oxygen-containing functionalities using hazardous chemicals like hydrazine as a reducing agent. The process of chemical reduction contains crucial and lengthy steps of chemical reactions, tiresome washing cycles, and drying of residue. Moreover, the use of poisonous and explosive reducing agents in the redox process makes it non-eco-friendly. Hence, functionalized GO using eco-friendly reduction methods is necessary in biosensing applications. Copper oxide (CuO) can be used to functionalize GO as a catalyst because of its electrochemical catalytic property (Khan et al. 2021). In an electrolysis process, Cu (II) oxidizes Cu (III) by gaining electrons in the redox reaction. During the oxidization process, glucose gets converted into gluconolactone (Zhang et al. 2020; Wang et al. 2014). Hence, an efficient electron transfer in glucose oxidation can be predicted through excellent support of GO to CuO. In the present work, an enzyme-less amperometric glucose biosensor using graphene oxide (GO) and copper oxide (CuO) nanocomposites was successfully prepared using a fluorine-doped tin oxide (FTO) substrate. The prepared sensor electrode exhibits exclusive properties such as large superficial area, good catalytic activity, and excellent electrical conductivity. Moreover, the electrode provides improved sensitivity, low detection limit, and excellent recovery in human serum compared to previously reported literature. Furthermore, the preparation and detection procedures are simple, fast, less time consuming, and cost effective.


Natural graphite powder (S.Aldrich, 99.99%), potassium permanganate (KMnO4, 99%), hydrogen peroxide (H2O2, 30%), sulfuric acid (H2SO4, 99.99%), hydrochloric acid (HCl, 30%), phosphoric acid (H3PO4,85%), copper oxide (CuO, 99.9%), polyvinyl alcohol (PVA,99%), fluorine-doped tin oxide (FTO) substrates, D (+) glucose, dopamine, l-ascorbic acid, D (−) fructose, lactose were purchased from Qualigens Fine Chemicals, India. Deionized (DI) water was purchased from Sharad Agencies, India. The phosphate buffer solution (PBS) was prepared in the laboratory using the standard method. All the chemicals are of analytical research (AR) grade and used without further purification.

Synthesis of graphene oxide

Graphene oxide was synthesized using an earlier reported method (Marcano et al., 2010). Graphite powder and NaNO3 in 2:1 proportion were mixed with concentrated H2SO4 and H3PO4 in the ratio of 9:1 (wt %). Fifteen grams of KMnO4 was slowly added while stirring followed by the addition of the required amount of H2O2. If the mixture turns into bright yellow color, this represents a great oxidation level of GO. In order to eradicate SO42− ions, the solution was repeatedly washed and the brownish black precipitate was collected.

Synthesis of GO/CuO nanocomposites

Initially, 0.5 g GO and 0.2 g CuO were dissolved in DI and the solution was constantly stirred for half an hour by raising its temperature to 100 °C. Fifty milliliters of NaOH solution was slowly added to it. The temperature of the solution was retained for 10 min and allowed to cool. The GO/CuO composite was collected after evaporation.

Preparation of GO/CuO nanocomposite electrode

Ten milligrams of the GO/CuO nanocomposite was dispersed in 5 ml of DI water and 5 μl of PVA using bath sonication for half an hour. Then, 10 μl of the suspension was drop casted on the previously cleaned FTO substrate. The working area of the electrode was 1 cm2. The electrode was annealed at 250 °C.

Mechanism of GO/CuO/FTO glucose sensing

A new decorum for the synthesis of the GO/CuO nanocomposite by in situ hydrothermal reduction of GO and CuO nanobelt formation is developed as shown in Fig. 1. With the existence of NaOH, DI water assists in the growth of Cu (OH)2 nanograin morphology on the GO surface. The epitaxial growth process caused the formation of CuO nanobelts. The augmented temperature of the above reaction mixture leads to the formation of a fiber-like GO/CuO nanostructure. A glucose sensing performance of the GO/CuO/FTO-modified electrode was studied in the presence of phosphate buffer solution (PBS:7.4 pH).

Fig. 1
figure 1

Glucose sensing mechanism of GO/CuO/FTO electrode

Normally, when glucose disperses in PBS, it creates d-gluconolactone and hydrogen peroxide (H2O2). It further generates d-gluconate with H+ ions due to electrooxidation of glucose at grain boundaries of CuO. Hence, gluconolactone is the key product accountable for oxidation that hydrolyzes gluconic acid. H+ ions lessen the pre-absorbed oxygen by discharging electrons which decrease the barrier potential between successive grains and lifts the electrical conductivity (Wu et al., 2010).

$$ {\displaystyle \begin{array}{c} CuO+{H}_2O\to Cu{(OH)}_4^{-}+{e}^{-}\\ {}{Cu}^{3+}+ glucose+{e}^{-}\to gluconolactone+{Cu}^{2+}\\ {} gluconolactone\ \overset{hydrolysis}{\to }\ gluconic\ acid\end{array}} $$

Structural and optical characterization

For structural characterization, an XRD analysis for the synthesized GO and GO/CuO powder was done with Bruker D8-Advanced Diffractometer using Cu Kα radiation (λ = 0.154 nm) from the range of 5 to 85° with a scanning rate of 2°/min. Surface morphology and elemental composition of GO and GO/CuO samples were characterized FESEM: FEI Nova NanoSEM 450, Raman analysis was done using a micro-Raman spectrometer (Jobin-Yuon HR 800 UV) using a He–Ne (633 nm) laser excitation source. XPS analysis of GO and CuO was carried out by Multifunctional XPS (PHI ulvac probe III Scanning Microprobe). For the FTIR study, FTIR-6100 spectrometer (JASCO) in the transmission (T) mode in the wavenumber range 4000–400 cm−1 was taken.

Electrochemical measurement

Wonatech potentiostat was used for voltametric and amperometric measurements for glucose detection with a three-electrode system which comprises GO/CuO/FTO as a working electrode along with platinum as counter and Ag/AgCl as reference electrode. The electrocatalytic performance of the electrode was studied using linear sweep voltammetry (LSV) and chronoamperometry.

Results and discussion

XRD analysis

XRD pattern of GO and GO/CuO nanocomposite is presented in Fig. 2.

Fig. 2
figure 2

XRD pattern of a GO and b GO/CuO

The reflection peak which occurred at (002) at 11.92° shows the occurrence of oxygen functionalities on the GO surface with an interlayer basal space of 0.79 nm (Tong et al. 2011). The peak (100) at 42.56° suggested the static disorder of GO. In the case of the GO/CuO nanocomposite, the reflection peak (002) at 24.04° showed a shift with basal spacing of 0.37 nm. The fall in basal space suggested that GO was converted to rGO (Dhara et al. 2015). All peaks of CuO are in good agreement with the JCPDS file: 48-1548, which confirms a high degree of purity and crystallinity of CuO.

FESEM and EDS analysis

The surface morphology of prepared GO and GO/CuO nanocomposites was characterized by FESEM as shown in Fig. 3.

Fig. 3
figure 3

FESEM of GO and GO/CuO

Graphene oxide showed a thin wrinkled paper-like structure. In the GO/CuO nanocomposite, high-density nanofibers of sizes in the range of 70–200 nm and several nanometers in length were distributed on the graphene layer. The formation of GO/CuO can be explained as follows.

Due to the increase in temperature during the synthesis, quick evaporation of water molecules caused a contraction of CuO nanoparticles and GO sheets. GO was thermally reduced to modified graphene. The graphene 2D material has a strong hydrophobic center and hydrophilic surrounding (Georgakilas et al. 2016). It was noted that the active GO surface lowers the internal energy.

Furthermore, CuO has a monoclinic nature and excellent thermal conductivity. Therefore, CuO nanoparticles together with GO formed a fiber-like structure. The distribution of chemical elements present in the composite was examined using the energy dispersive spectrum (EDS) as shown in Fig. 4.

Fig. 4
figure 4

EDS of GO and GO/CuO

FTIR analysis

FTIR spectra for GO and GO/CuO nanocomposite is as shown in Fig. 5.

Fig. 5
figure 5

FTIR of a GO and b GO/CuO

The bending and stretching modes of O-H groups were represented as broad spectra present around 3203 cm−1 and the peak at 1734cm−1 Showed C=O stretching vibrations on the surface of GO. The C-O stretching vibrations were assigned to the peak at 1054 cm−1 (Xu Zhu et al. 2012). This surface functional group exhibited the probable bonding of CuO on the GO surface. The peaks at low frequencies below 1000 cm−1 in GO/CuO spectra could be ascribed to Cu-O-Cu and Cu-O-C vibrations (Q Chen et al. 2012). This demonstrated the chemical composition of GO/CuO where GO was partially reduced in presence of cuprous ions.

Raman spectroscopy

Raman analysis of the GO-CuO nanocomposite is presented in Fig. 6.

Fig. 6
figure 6

Raman Spectrum of a GO and b GO/CuO nanocomposite

The fundamental vibrations of GO and GO/CuO were observed in the sequence of 1200 to 1700 cm−1. The D (disorder bands) and G (tangential bands) were formed at breathing mode A19 symmetry and first-order scattering of E29 photons by Sp2 carbon (C-C bond) respectively (AC Ferrari et al. 2006). A broad 2D band of GO with a higher wavenumber was located at 2918 cm−1 confirming the multilayered nature of GO. The 2D band of GO/CuO predicted the reduction of GO to rGO caused GO/CuO to stack. The ID/IG ratio of GO/CuO was slightly decreased specifiying that there were some structural changes that occurred during thermal reduction.

XPS analysis

XPS spectrum of GO and GO/CuO nanocomposite is presented in Fig. 7. The peaks centered at C, O, and CuO are core-level regions associated with C1s, O1s, and Cu2p peaks respectively.

Fig. 7
figure 7

XPS spectrum of a GO b GO/CuO

Deconvoluted spectrum of GO/CuO

A deconvoluted spectrum of GO/CuO is shown in Fig. 8.

Fig. 8
figure 8

Deconvoluted spectrum of GO/CuO a C1s, b O1s, and c Cu2p

Carbon in the nanocomposite was identified by the high-resolution spectrum of C1s. The major peak at 283.5 eV exhibited sp2 (C-C, C=C) bonding and the shoulder peaks revealed C=O and O-C=O bonding. The chemical bonding states of C-OH and C-O-C were located at characteristic peaks 286.8 eV. The presence of CuO and Cu2O was confirmed using high-resolution O1s spectra. The characteristic peaks observed at 530.2 eV belong to Cu-O, -C=O bonding in GO/CuO. The peak shown at 532.9 eV was assigned to oxygen present in GO. The XPS spectrum of Cu 2p showed a peak at 932.5 eV for Cu 2p3/2 and confirmed the presence of CuO as a catalyst. A binding energy peak at 952.2 eV was allocated to Cu 2p1/2 (Wu J et al. 2018).

Electrochemical measurements

Linear sweep voltammogram to study the electrocatalytic activity of the modified electrodes is represented in Fig. 9.

Fig. 9
figure 9

LSV of a FTO, b GO/FTO, c CuO/FTO, and d GO/CuO/FTO at 100 mv/s scan rate

The electrodes were scanned at 100 mv/s in presence of 5 mM D (+) glucose. There was no signal response detected for bare FTO (curve a). GO/FTO (curve b) and CuO/FTO (curve c) showed a small increase in background current due to the large superficial area of GO and electrocatalytic capability for glucose oxidation of CuO respectively. A well-defined glucose oxidation peak was observed for GO/CuO/FTO (curve d) in the presence of 5 mM glucose at + 0.6 V. This indicates that the GO/CuO/FTO composite electrode is necessary to obtain high sensitivity and better electrocatalytic activity. During the oxidation process, CuO was oxidized to CuOOH and glucose to gluconolactone by Cu (III) (Wei H et al. 2009).

Further, the electrochemical effect on GO/CuO/FTO with a variable scan rate was examined using LSV as shown in Fig. 10. The electrode was scanned from 10 to 300 mv/s (a to h) in PBS electrolyte in the presence of 5 mM D (+) glucose. The oxidation occurred at + 0.6 V for each scan and the oxidation peak current was increased with an increase in scan rate. This revealed that electrochemical reaction takes place on GO/CuO nanocomposite demonstrating its ability towards enzyme sensing (Wang et al. 2012). Hence, further electrochemical and amperometric measurements were carried out at the optimized potential of + 0.6 V vs. Ag/AgCl.

Fig. 10
figure 10

LSV of GO/CuO/FTO electrode with variable scan rate

Amperometric measurements

The amperometric response of the GO/CuO/FTO electrode was measured and presented in Fig. 11a. The proposed electrode revealed that the electron transport between PBS solution and electrode in the redox process is enhanced due to the high electrical conductivity and electrocatalytic activity of the GO/CuO nanocomposite (Hsu et al. 2012). The calibration curve in Fig.11b displayed two linear ranges corresponding to low glucose concentration (0.1–1 mM) and high concentration (1–10 mM).

Fig. 11
figure 11

a Chronoamperometric response of GO/CuO/FTO. b Calibration curve

The corresponding linear regression equations were Ip = 424.95c + 569.22 with R2 = 0.9984 (N = 9) and Ip = 27.666c + 826 with R2 = 0.9963 (N = 9) respectively. For low glucose concentration the sensitivity was 1274.8 μA mM−1cm−2 (S/N = 3) whereas for high concentration was 830 μA mM−1cm−2 along with a low detection limit of 0.13 μM. A comparative study of performance of various glucose biosensors is displayed in Table 1.

Table 1 Comparison of the enzyme-free GO-based glucose sensor with its composites

Selectivity, stability, and reproducibility

The selectivity and stability of the GO/CuO/FTO sensor electrode are presented in Fig. 12.

Fig. 12
figure 12

a Selectivity and b stability of GO/CuO/FTO glucose sensor

The biological range of glucose concentration in human serum is 3–8 mM, which is quite greater compared to other interfering substances like ascorbic acid, dopamine, etc. Hence, the electrode amperometric responses were examined with 1 mM D (+) glucose with the abovementioned interfering species (0.1 mM) in PBS (7.4) solution. It was observed that the glucose sensing ability of the proposed sensor was unaffected by the interfering substances. The reproducibility of the sensor was studied using 10 similar sensors. The current response was observed for 1 mM glucose concentration for each sensor. RSD of 2.7% confirms the significant reproducibility. The aging effect (stability) of the samples was also tested periodically for 30 days with of 1 mM glucose concentration and an RSD of 2.64% was achieved. This demonstrates that the GO/CuO/FTO electrode has good repeatability, reproducibility, and stability as a glucose biosensor.

Determination of glucose concentration in human serum

The blood glucose level in human serum samples was determined using the proposed glucose biosensor. The human serum samples were received from a standard pathology laboratory. The samples were diluted (100 folds) using the standard dilution method before analysis. The results obtained using the GO/CuO/FTO sensor were compared with the certified values received from the pathology laboratory (Table 2). The average recovery rate was 99.17% along with an RSD of 1.58% which assured the reliability and applicability of the GO/CuO/FTO sensor to determine the glucose concentration in a real sample.

Table 2 Analysis of the glucose sensing performance of GO/CuO/FTO electrode


The GO/CuO/FTO electrode was successfully prepared using the hydrothermal method and employed as a glucose biosensor. The developed sensor has numerous advantages, such as a simple, eco-friendly method, quick detection with LOD 0.13 μM, fast analysis, high sensitivity of 1274.8 μA mM−1 cm−2 (S/N = 3), good selectivity, excellent reproducibility, and good stability. The RSD of 1.58% obtained in human serum samples supports the reliability of the developed biosensor

Availability of data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.



Graphene oxide


Copper oxide


Fluorine-doped tin oxide


Polyvinyl alcohol


Phosphate buffer solution


Linear sweep voltammetry


Limit of detection


Relative standard deviation


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Authors and Affiliations



Medha Gijare: First author, Conceptualization, Methodology, Data curation, Writing—original draft preparation, Investigation. Sharmila Chaudhari: Supervision and review. Satish Ekar: Visualization, Investigation, Supervision. Anil Garje: Corresponding author, Writing—reviewing and editing, Validation. All authors read and approved the final manuscript.

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Correspondence to Anil Garje.

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Gijare, M., Chaudhari, S., Ekar, S. et al. A facile synthesis of GO/CuO-blended nanofiber sensor electrode for efficient enzyme-free amperometric determination of glucose. J Anal Sci Technol 12, 40 (2021).

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  • Graphene oxide
  • Enzyme free
  • Glucose
  • Biosensor