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A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye

Abstract

Background

In this paper, a simple, enzyme-free, label-free fluorescence, high sensitivity logic gate hairpin aptasensor was developed for adenosine triphosphate (ATP) detection based on graphene oxide (GO) and PicoGreen dye.

Methods

Using single-strand deoxyribonucleic acid (DNA) and adenosine triphosphate (ATP) as input signal and fluorescence signal as output signal, if single-strand DNA (DNA-L), single-strand DNA (DNA-S), and ATP were present at the same time, one segment of DNA-L formed a hairpin ring with ATP, and the other segment of DNA-L formed a completely complementary hairpin stem with DNA-S. The hairpin DNA was detached from the GO surface, and PicoGreen dye was embedded into the hairpin stem, and the fluorescence signal was enhanced. The molecular logic gate was constructed through the establishment of logic histogram, logic circuit, truth table, and logic formula. The biosensor-related performances including sensitivity, selectivity, and linearity were investigated, respectively.

Results

We have successfully constructed a AND logic gate. The detection limit of ATP is 138.0 pmol/L (3σ/slope) with detection range of 50–500 nmol/L (R2 = 0.98951), and its sensitivity is 4.748 × 106–6.875 × 108 a.u. (mol/L)−1.

Conclusions

The logic gate hairpin aptamer sensor has the advantages of high sensitivity, low detection limit, and low cost, and can be successfully applied to the detection of adenosine triphosphate (ATP) in actual human urine samples.

Introduction

Adenosine triphosphate (ATP) is associated with hypoglycemia, Parkinson’s disease, hypoxia, and ischemia (Aw and Jones 1985; Agardh et al. 1978; Katsura et al. 1993). Consequently, the study of ATP detection method has important clinical significance. With high selectivity and sensitivity (Xiao et al. 2015), fluorescent biosensors have been widely used in the detection of nucleic acids, ions, proteins, and organic molecules (Li et al. 2017; Qiu et al. 2013; Wang et al. 2015).

The signal amplification and background signal suppression are two main strategies for improving DNA fluorescence sensors sensitivity. Common methods based on signal amplification can effectively increase sensor sensitivity, including rolling circle replication (RCR) (Zhou et al. 2015), strand displacement amplification (SDA) (Chen et al. 2016; Wang et al. 2013; Wei et al. 2016; Wang et al. 2017), and DNA enzymes (Zeng et al. 2015; Lu et al. 2010; He et al. 2017; Ning et al. 2017), etc. But the strategy used in these literatures requires DNA enzymes. The price of DNA enzyme is relatively high in practical application, and it is easily affected by pH and reaction temperature.

Compared with signal amplification strategies used in above literatures, suppressing the background signal strategies can design sensor with a simpler structure and needs a shorter reaction time (Liu et al. 2015; Lv et al. 2015). The quencher, such as gold nanoparticles (Jin et al. 2009; Yin et al. 2010), graphene oxide (GO) (Liu et al. 2015; Lv et al. 2015; Luo et al. 2018), metal-organic frameworks (Hai et al. 2018), and magnetic nanoparticles (Saberi et al. 2018), can inhibit the background signal to increases sensor sensitivity. But these approaches usually involve fluorescence-labeled probe, resulting in relatively high cost of detection. Therefore, label-free fluorescence strategies are favored by scholars. Fluorescent dye probes, such as these based on dyes thiazole orange (TO) (Kang et al. 2016), PicoGreen (Dragan et al. 2010; Lv et al. 2013) and SYBR Green I (Pu et al. 2012), and Thioflavin T (Liu et al. 2017; Song et al. 2017), have proved to be effective label-free fluorescence methods. However, neither signal suppression nor signal amplification was applied in most of these label-free fluorescence methods. In this work, we investigated the use of GO and PicoGreen dye to construct a biosensor for ATP based on enzyme-free and label-free fluorescence.

Molecular computer is of substantial significance in nanotechnology, medicine, and biotechnology. DNA logic gates can perform Boolean operations and are the hardware basis of DNA computers. Till now, many DNA logic gates, such as INH + NINH, INHIBIT, AND-NOT-AND-OR, and AND (Lv et al. 2015; Voelcker et al. 2008; Zhang et al. 2018a; Li et al. 2011), have been established. Although many molecular logic gates have been developed, there is still a need to construct logic gates that are more simple, sensitive, and less expensive. In this work, we constructed an AND logic gate based on label-free fluorescence using GO and PicoGreen, which is enzyme-free, simple, and highly sensitive.

Experimental

Reagents

The reagents and DNA sequences are shown in Tables 1 and 2, respectively. All reagents were performed in buffer solution (10 mM Tris, 10 mM MgCl2, 50 mM NaCl, pH 7.5, and deionized water). DNA-L and DNA-S were heated for 5 min at 95 °C, and were then stored at 4 °C in a refrigerator, respectively. PicoGreen dye (200-fold dilution) and GO (100-fold dilution) were stored in a refrigerator at 4 °C. In all experiments, the volumes of DNA-L, DNA-S, PicoGreen dye, GO, and buffer solution were 30 μL, 30 μL, 20 μL, 10 μL, 1800 μL, respectively.

Table 1 Reagents
Table 2 DNA sequences

Before each experiment, the reagents and buffer were placed in a 2-mL sample tube, and the reaction mixture was kept for at least 24 h at room temperature.

Apparatus

Reaction times and fluorescence spectra were tested by a fluorescence spectrometer (Shimadzu, Osaka, Japan, Model: RF-6000). The wavelengths of emission and excitation used in fluorescence spectra were 480 nm and 520 nm, respectively. The emission wavelength used in the scanning spectrum was 480 nm.

Recovery of ATP in urine

Urine samples were provided by volunteers and centrifuged for 3 min at 13,000 r/min. Then, the supernatant of the centrifuged sample was diluted to 10 times with pH value of 7.5. The ATP was added in diluted samples at concentrations of 100 nM, 300 nM, and 500 nM, respectively. Each sample measurement was repeated in triplicate to compute the recovery.

Results and discussion

The principle of a logic gate hairpin aptasensor

When the emission spectrum of the donor fluorescence molecule overlaps with the absorption spectrum of the receptor fluorescence molecule, and the distance between the two molecules is less than a certain distance, the fluorescence energy will transfer from the donor to the receptor. If the fluorescence quantum yield of the acceptor is zero, the fluorescence resonance energy transfer (FRET) will lead to fluorescence extinction. In this work, PicoGreen fluorescence molecule was used as energy donor and GO as energy receptor. Single-stranded DNA was adsorbed on the surface of GO through π-π stacking, fluorescence resonance energy transfer (FRET) occurred between PicoGreen dye combined with single-stranded DNA and GO, and fluorescence was quenched. But double-stranded DNA with perfect pairing did not have this property (Zhang et al. 2018a; Li et al. 2011; Zhang et al. 2018b; Zhang et al. 2018c). Scheme 1 exhibits the principle of an aptasensor for ATP detection. In the presence of a single-strand DNA (DNA-L), fluorescence was quenched because DNA-L was adsorbed on the surface of GO, resulting in FRET. When ATP was added, the ATP aptamer (the red fragment in DNA-L) formed a hairpin with ATP, and another fragment (the blue fragment in DNA-L) adsorbed on GO, resulting in fluorescence quenching. When a single-strand (DNA-S) was added, it paired with blue fragment in DNA-L to form complementary hairpin double-stranded DNA. The hairpin double-stranded had a weak binding force with GO, and it detached from the GO. The distance between PicoGreen embedded in double-stranded DNA and GO increased, and FRET could not occur between PicoGreen and GO. The fluorescence was significantly enhanced, thus achieving the purpose of ATP detection. If DNA-L, ATP, and DNA-S were taken as input signals and fluorescence as output signals, this principle conformed to AND logic operation law. In this principle, the PicoGreen dye was used to construct a label-free and enzyme-free hairpin aptasensor, and the GO inhibited the fluorescence background signal generated by the binding of incomplete paired hairpin double stranded DNA, which was beneficial to improve the sensitivity of the sensor.

Scheme 1
scheme1

The principle of a logic gate hairpin aptasensor for ATP detection

Mechanism feasibility verification

Figure 1 exhibits that the fluorescence signals (a- to g-curve vs. b-curve) were relatively weak because of the adsorption of single-stranded DNA on the GO surface, leading to FRET. The h-curve fluorescence signal was obviously enhanced. Because ATP aptamer segment in DNA-L formed a hairpin structure with ATP, and the A-based segment in DNA-L was completely paired with DNA-S, resulting in double-stranded hairpin DNA shedding from GO surface. PicoGreen interacted with double-stranded DNA (DNA-S + DNA-L) to produce more obvious fluorescence signals. It shows that the 3:1 logic gate is feasible for use in detection of ATP.

Fig. 1
figure1

Fluorescence spectrum: a PicoGreen dye, GO. b PicoGreen dye, GO, DNA-L, 100 μL of 10 mM ATP. c PicoGreen dye, GO, 100 μL of 10 mM ATP. d PicoGreen dye, GO, DNA-L. e PicoGreen dye, GO, DNA-S. f PicoGreen dye, GO, DNA-L, DNA-S. g PicoGreen dye, GO, 100 μL of 10 mM ATP, DNA-S. h PicoGreen dye, GO, 100 L 10 mM ATP, DNA-L, DNA-S

The purpose of the following experiments was to further verify the correctness of the principle. Figure 2A shows that when GO was added to mixed solutions, the fluorescence signal rapidly decreased because of the adsorption of single-stranded DNA on the GO surface, leading to FRET. This result was consistent with the result of the a-curve (see Fig. 1). Figure 2B demonstrates that the fluorescence signal is enhanced in the presence of DNA-L, DNA-S, and ATP. Because ATP aptamer segment in DNA-L formed a hairpin structure with ATP and the A-based segment in DNA-L was completely paired with DNA-S to form dsDNA (DNA-L+DNA-S), falling off the GO surface. And then PicoGreen interacted with dsDNA (DNA-S + DNA-L) to produce more obvious fluorescence signal which also demonstrates the result of h-curve (see Fig. 1). Figure 3c shows that the fluorescence signal does not change in the presence of DNL-L and DNA-S. Because DNL-L and DNA-S are paired to form double stranded DNA (DNL-L + DNA-S) with sticky ends, which were adsorbed on the GO surface, resulting in FRET and fluorescence quenching. These results further prove that of f-curve shown in Fig. 1. In summary, the results in Fig. 2 are consistent with those described in Fig. 1, which further verifies that the principle of the 3:1 logic gate is feasible.

Fig. 2
figure2

Reaction time: A (a) PicoGreen dye, DNA-L, DNA-S, (b) GO. B (a) PicoGreen dye, GO, 100 μL of 10 mM ATP, DNA-L, (b) DNA-S. C (a) PicoGreen dye, GO, DNA-L, (b) DNA-S

Fig. 3
figure3

a Logic histogram. b Circuit diagram

Construction of DNA logic

If DNA-L, DNA-S, or ATP exits, the input signal of logic gate is 1, if not, the input signal is 0, labeled as (DNA-L, ATP, DNA-S). Meanwhile, when the fluorescence change, F, is greater than 50 a.u., the output signal of logic gate is 1, otherwise it is 0. The fluorescence change value is F = FF0, where F0 is the a-curve peak value, and F is the other curves (b- to h-curve) peak value (see Fig. 1). According to the result of Fig. 1, the logic bar chart is drawn, as shown in Fig. 3a. Figure 3a exhibits that DNA-L, ATP, and DNA-S coexist (1,1,1), the fluorescence intensity changes significantly. Using to the results of Fig. 3a, the DNA circuit is constructed as shown in Fig. 3b.

According to Fig. 3 results, the truth table is constructed (Table 3). Using the truth Table 3, the following can be deduced:

$$ Out={I}_1\cdot {I}_2\cdot {I}_3 $$
(1)
Table 3 Truth table

A three input single output (3:1) AND logic gate is drawn by formula (1), as shown in Fig. 4.

Fig. 4
figure4

Three input single output (3:1) AND logic gate

Linearity and sensitivity

All the reagents were stored at 4 °C, because the conformation of DNA and ATP were stable at low temperature, which effectively improved the stability of the sensor. Figure 5a exhibits the spectral curves of different ATP concentrations. The fluorescence intensity of a-curve is taken as F0, and the other curve (b-, c-, d-, e-, f-, g-curve) peak is taken as F. Figure 5b is calculated based on the result of Fig. 5a. The detection range was from 50 nM to 500 nM. The fitting linear equation was y = 0.00455x + 0.13369 (R2 = 0.98951) with the detection limit of 138.0 pmol/L (3σ/slope). The results in Table 4 clearly exhibit that the proposed method has lower detection limits, sufficient linearity, and higher sensitivity compared with most other documents. The sensitivity is shown in Fig. 6 with a range of 4.748 × 106 a.u.(mol/L)−1 − 6.875 × 108 a.u. (mol/L) −1.

Fig. 5
figure5

a Spectral curves of the various ATP concentrations. b Linear fitting

Table 4 Comparison of methods
Fig. 6
figure6

Sensitivity of sensors

Specificity

In order to evaluate the specificity of the sensor, the similar molecules (CTP, UTP, and GTP) was tested by the proposed method. All reagents were stored in refrigerator at 4 °C to maintain the conformation of DNA and the performance of reagents (ATP, CTP, UTP, and GTP). Figure 7 exhibits that compared with the similar molecules (CTP, UTP, and GTP), fluorescence signal increased significantly in the presence of the target ATP. In addition, there was no significant difference between the fluorescence signals caused by mixture and ATP alone. These results clearly prove that this method has enough specificity.

Fig. 7
figure7

The selectivity: ATP, UTP, CTP, and GTP were 510 nM respectively; mixture sensing system consisted of 510 nM ATP, 510 nM UTP, 510 nM CTP, and 510 nM GTP

Detection of ATP in real sample

The purpose of recovery test is to verify the practical application performance of the sensor. Table 5 shows that the 3:1 AND logic gate has good recoveries in the range of 97.54–108.04%, indicating that the proposed method exhibits immense potential for ATP detection in human urine.

Table 5 ATP recovery test

Conclusions

We have developed a logic gate hairpin aptasensor for sensitivity detection of ATP. This 3:1 AND logic gate has many advantages. First, the GO suppresses the background signal to improve sensitivity. Second, the proposed assay does not require enzymes or fluorescent labeling, thus reducing the cost of analysis. Third, this method has sufficient specificity for the detection of ATP, and the detection range was from 50 to 500 nmol/L with the detection limit of 138.0 pmol/L. Finally, the recommended method successfully detects ATP in urine. As a result of above advantages, the proposed strategy can also be applied to the detection of other ions and proteins.

Availability of data and materials

Research data have been provided in the manuscript and supporting information.

Abbreviations

GO:

Graphene oxide

FRET:

Fluorescence resonance energy transfer

DNA:

Deoxyribonucleic acid

dsDNA:

Double-stranded deoxyribonucleic acid

DNA-L:

Single-stranded deoxyribonucleic acid with the number L

DNA-S:

Single-stranded deoxyribonucleic acid with the number S

ATP:

Adenosine triphosphate

UTP:

Uridine triphosphate

CTP:

Cytidine triphosphate

GTP:

Guanosine triphosphate

References

  1. Agardh CD, Folbergrova J, Siesjo BK. Cerebral metabolic changes in profound, insulin-induced hypoglycemia, and in the recovery period following glucose administration. J Neurochem. 1978;31:1135–42.

    CAS  Article  Google Scholar 

  2. Aw TY, Jones DP. ATP concentration gradients in cytosol of liver cells during hypoxia. Am J Physiol. 1985;249:C385–92.

    CAS  Article  Google Scholar 

  3. Chen HG, Ren W, Jia J, Feng J, Gao ZF, Li NB, Luo HQ. Fluorometric detection of mutant DNA oligonucleotide based on toehold strand displacement-driving target recycling strategy and exonuclease III-assisted suppression. Biosens Bioelectron. 2016;77:40–5.

    Article  Google Scholar 

  4. Dragan AI, Casas-Finet JR, Bishop ES, Strouse RJ, Schenerman MA, Geddes CD. Characterization of PicoGreen Interaction with dsDNA and the Origin of Its Fluorescence Enhancement upon Binding. Biophys J. 2010;99:3010–9.

    CAS  Article  Google Scholar 

  5. Gu Y, Li J, Qian K, Zhang Z, Wang S, Wang J. Integrated dual-signal aptasensor based on magnet-driven operations and miniaturized analytical device for on-site analysis. Sensors Actuators B: Chemical. 2020;310:127856.

    CAS  Article  Google Scholar 

  6. Hai X-M, Li N, Wang K, Zhang Z-Q, Zhang J, Dang F-Q. A fluorescence aptasensor based on two-dimensional sheet metal-organic frameworks for monitoring adenosine triphosphate. Anal Chim Acta. 2018;998:60–6.

    CAS  Article  Google Scholar 

  7. He S, Qu L, Tan Y, Liu F, Wang Y, Zhang W, Cai Z, Mou L, Jiang Y. A fluorescent aptasensor with product-triggered amplification by exonuclease III digestion for highly sensitive ATP detection. Analytical Methods. 2017;9:4837–42.

    CAS  Article  Google Scholar 

  8. Jin Y, Li H, Bai J. Homogeneous selecting of a quadruplex-binding ligand-based gold nanoparticle fluorescence resonance energy transfer assay. Anal Chem. 2009;81:5709–15.

    CAS  Article  Google Scholar 

  9. Kang BH, Gao ZF, Li N, Shi Y, Li NB, Luo HQ. Thiazole orange as a fluorescent probe: Label-free and selective detection of silver ions based on the structural change of i-motif DNA at neutral pH. Talanta. 2016;156-157:141–6.

    CAS  Article  Google Scholar 

  10. Katsura K, Folbergrova J, Bengtsson F, Kristian T, Gido G, Siesjo BK. Recovery of mitochondrial and plasma membrane function following hypoglycemic coma: coupling of ATP synthesis, K+ transport, and changes in extra- and intracellular pH. J Cereb Blood Flow Metab. 1993;13:820–6.

    CAS  Article  Google Scholar 

  11. Lee JD, Cang J, Chen Y-C, Chen W-Y, Ou C-M, Chang H-T. Detection of adenosine 5 '-triphosphate by fluorescence variation of oligonucleotide-templated silver nanoclusters. Biosens Bioelectron. 2014;58:266–71.

    CAS  Article  Google Scholar 

  12. Li J, Huang Y-Q, Qin W-S, Liu X-F, Huang W. An optical-logic system based on cationic conjugated polymer/DNA/intercalating dyes assembly for label-free detection of conformational conversion of DNA i-motif structure. Polymer Chemistry. 2011;2:1341–6.

    CAS  Article  Google Scholar 

  13. Li Q, Li S, Chen X, Bian L. A G-quadruplex based fluorescent oligonucleotide turn-on probe towards iodides detection in real samples. Food Chem. 2017;230:432–40.

    CAS  Article  Google Scholar 

  14. Liu H, Ma C, Ning F, Chen H, He H, Wang K, Wang J. A facile label-free G-quadruplex based fluorescent aptasensor method for rapid detection of ATP. Spectrochimica Acta Part a-Molecular And Biomolecular Spectroscopy. 2017;175:164–7.

    CAS  Article  Google Scholar 

  15. Liu X, Yang Y, Hua X, Feng X, Su S, Huang Y, Fan Q, Wang L, Huang W. An improved turn-on aptasensor for thrombin detection using split aptamer fragments and graphene oxide. Chinese J Chem. 2015;33:981–6.

    CAS  Article  Google Scholar 

  16. Lu C-H, Li J, Lin M-H, Wang Y-W, Yang H-H, Chen X, Chen G-N. Amplified Aptamer-Based Assay through Catalytic Recycling of the Analyte. Angewandte Chemie-International Edition. 2010;49:8454–7.

    CAS  Article  Google Scholar 

  17. Lu L, Qian Y, Wang L, Ma K, Zhang Y. Metal-enhanced fluorescence-based core-shell Ag@SiO(2) nanoflares for affinity biosensing via target-induced structure switching of aptamer. ACS Appl Mater Interfaces. 2014;6:1944–50.

    CAS  Article  Google Scholar 

  18. Luo, J., Shen, X., Li, B., Li, X. & Zhou, X. Signal amplification by strand displacement in a carbon dot based fluorometric assay for ATP. Microchim Acta. 2018;185. https://webvpn.hainanu.edu.cn/https/77726476706e69737468656265737421fcfe4f976923784277068ea98a1b203a54/article/10.1007%2Fs00604-018-2931-2.

  19. Lv H, Li S, Liu Y, Wang G, Li X, Lu Y, Wang J. A reversible fluorescent INHIBIT logic gate for determination of silver and iodide based on the use of graphene oxide and a silver-selective probe DNA. Microchimica Acta. 2015;182:2513–20.

    CAS  Article  Google Scholar 

  20. Lv Z, Liu J, Zhou Y, Guan Z, Yang S, Li C, Chen A. Highly sensitive fluorescent detection of small molecules, ions, and proteins using a universal label-free aptasensor. Chemical Communications. 2013;49:5465–7.

    CAS  Article  Google Scholar 

  21. Ma Y, Geng F, Wang Y, Xu M, Shao C, Qu P, Zhang Y, Ye B. Novel strategy to improve the sensing performances of split ATP aptamer based fluorescent indicator displacement assay through enhanced molecular recognition. Biosensors Bioelectronics. 2019;134:36–41.

    CAS  Article  Google Scholar 

  22. Mashhadizadeh MH, Naseri N, Mehrgardi MA. A simple non-enzymatic strategy for adenosine triphosphate electrochemical aptasensor using silver nanoparticle-decorated graphene oxide. J Iran Chemi Soc. 2017;14:2007–16.

    CAS  Article  Google Scholar 

  23. Ning Y, Wei K, Cheng L, Hu J, Xiang Q. Fluorometric aptamer based determination of adenosine triphosphate based on deoxyribonuclease I-aided target recycling and signal amplification using graphene oxide as a quencher. Microchimica Acta. 2017;184:1847–54.

    CAS  Article  Google Scholar 

  24. Pu W, Zhao H, Huang C, Wu L, Xua D. Fluorescent detection of silver(I) and cysteine using SYBR Green I and a silver(I)-specific oligonucleotide. Microchimica Acta. 2012;177:137–44.

    CAS  Article  Google Scholar 

  25. Qiu B, Zhang Y-S, Lin Y-B, Lu Y-J, Lin Z-Y, Wong K-Y, Chen G-N. A novel fluorescent biosensor for detection of target DNA fragment from the transgene cauliflower mosaic virus 35S promoter. Biosens Bioelectron. 2013;41:168–71.

    CAS  Article  Google Scholar 

  26. Saberi Z, Rezaei B, Khayamian T. A fluorescent aptasensor for analysis of adenosine triphosphate based on aptamer-magnetic nanoparticles and its single-stranded complementary DNA labeled carbon dots. Luminescence. 2018;33:640–6.

    CAS  Article  Google Scholar 

  27. Shahsavar K, Hosseini M, Shokri E, Ganjali MR, Ju H. A sensitive colorimetric aptasensor with a triplehelix molecular switch based on peroxidase-like activity of a DNAzyme for ATP detection. Analytical Methods. 2017;9:4726–31.

    CAS  Article  Google Scholar 

  28. Song Q, Wang R, Sun F, Chen H, Wang Z, Na N, Ouyang J. A nuclease-assisted label-free aptasensor for fluorescence turn-on detection of ATP based on the in situ formation of copper nanoparticles. Biosens Bioelectron. 2017;87:760–3.

    CAS  Article  Google Scholar 

  29. Voelcker, N.H., Guckian, K.M., Saghatelian, A. & Ghadiri, M.R. (2008) Sequence-addressable DNA logic. Small (Weinheim an der Bergstrasse, Germany), 4, 427-431.

  30. Wang G, Su X, Xu Q, Xu G, Lin J, Luo X. Antifouling aptasensor for the detection of adenosine triphosphate in biological media based on mixed self-assembled aptamer and zwitterionic peptide. Biosens Bioelectron. 2018;101:129–34.

    CAS  Article  Google Scholar 

  31. Wang K, Liao J, Yang X, Zhao M, Chen M, Yao W, Tan W, Lan X. A label-free aptasensor for highly sensitive detection of ATP and thrombin based on metal-enhanced PicoGreen fluorescence. Biosens Bioelectron. 2015;63:172–7.

    CAS  Article  Google Scholar 

  32. Wang X-P, Yin B-C, Wang P, Ye B-C. Highly sensitive detection of microRNAs based on isothermal exponential amplification-assisted generation of catalytic G-quadruplex DNAzyme. Biosens Bioelectron. 2013;42:131–5.

    Article  Google Scholar 

  33. Wang Y-M, Liu J-W, Duan L-Y, Liu S-J, Jiang J-H. Aptamer-based fluorometric determination of ATP by using target-cycling strand displacement amplification and copper nanoclusters. Microchimica Acta. 2017;184:4183–8.

    CAS  Article  Google Scholar 

  34. Wei Y, Zhou W, Li X, Chai Y, Yuan R, Xiang Y. Coupling hybridization chain reaction with catalytic hairpin assembly enables non-enzymatic and sensitive fluorescent detection of microRNA cancer biomarkers. Biosens Bioelectron. 2016;77:416–20.

    CAS  Article  Google Scholar 

  35. Xiao LL, Sun SG, Pei ZC, Pei YX, Pang Y, Xu YQ. A Ga3+ self-assembled fluorescent probe for ATP imaging in vivo. Biosens Bioelectron. 2015;65:166–70.

    CAS  Article  Google Scholar 

  36. Yang L, Liu X, Li L, Zhang S, Zheng H, Tang Y, Ju H. A visible light photoelectrochemical sandwich aptasensor for adenosine triphosphate based on MgIn2S4-TiO2 nanoarray heterojunction. Biosensors Bioelectronics. 2019;142:111487.

    CAS  Article  Google Scholar 

  37. Yin B-C, Zuo P, Huo H, Zhong X, Ye B-C. DNAzyme self-assembled gold nanoparticles for determination of metal ions using fluorescence anisotropy assay. Anal Biochem. 2010;401:47–52.

    CAS  Article  Google Scholar 

  38. Yu C-J, Wu S-M, Tseng W-L. Magnetite Nanoparticle-Induced Fluorescence Quenching of Adenosine Triphosphate-BODIPY Conjugates: Application to Adenosine Triphosphate and Pyrophosphate Sensing. Anal Chem. 2013;85:8559–65.

    CAS  Article  Google Scholar 

  39. Zeng S, Huang H, Huang Y, Liu X, Qin J, Zhao S, Chen Z-F, Liang H. Label-free and amplified colorimetric assay of ribonuclease H activity and inhibition based on a novel enzyme-responsive DNAzyme cascade. Rsc Advances. 2015;5:43105–9.

    CAS  Article  Google Scholar 

  40. Zhang J, Han J, Feng S, Niu C, Liu C, Du J, Chen Y. A label-free fluorescent DNA machine for sensitive cyclic amplification detection of ATP. Materials. 2018a;11. https://webvpn.hainanu.edu.cn/https/77726476706e69737468656265737421e7e056d22a347859300b86a1/1996-1944/11/12/2408.

  41. Zhang J, Yang C, Niu C, Liu C, Cai X, Du J, Chen Y. A label-free fluorescent and logic gate aptasensor for sensitive ATP Detection. Sensors (Basel). 2018c;18. https://webvpn.hainanu.edu.cn/https/77726476706e69737468656265737421e7e056d22a347859300b86a1/1424-8220/18/10/3281.

  42. Zhang, J., Zhang, S., Niu, C., Liu, C., Du, J. & Chen, Y. A label-free fluorescent DNA calculator based on gold nanoparticles for sensitive detection of ATP. Molecules. 2018d;23. https://webvpn.hainanu.edu.cn/https/77726476706e69737468656265737421e7e056d22a347859300b86a1/1420-3049/23/10/2494.

  43. Zhang X, Song C, Yang K, Hong W, Lu Y, Yu P, Mao L. Photoinduced regeneration of an aptamer-based electrochemical sensor for sensitively detecting adenosine triphosphate. Anal Chem. 2018b;90:4968–71.

    CAS  Article  Google Scholar 

  44. Zhou F, Li B, Ma J. A linear DNA probe as an alternative to a molecular beacon for improving the sensitivity of a homogenous fluorescence biosensing platform for DNA detection using target-primed rolling circle amplification. Rsc Advances. 2015;5:4019–25.

    CAS  Article  Google Scholar 

  45. Zhu Y, Hu X-C, Shi S, Gao R-R, Huang H-L, Zhu Y-Y, Lv X-Y, Yao T-M. Ultrasensitive and universal fluorescent aptasensor for the detection of biomolecules (ATP, adenosine and thrombin) based on DNA/Ag nanoclusters fluorescence light-up system. Biosens Bioelectron. 2016;79:205–12.

    CAS  Article  Google Scholar 

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Acknowledgements

All the authors are very grateful for the experimental equipment provided by Hainan University, as well as the financial support provided by Hainan Provincial Education Department and Hainan University famous teachers’ studio.

Funding

This work was supported by the Key Project of Research and Development in Hainan Province (Grant No. ZDYF2020038), the Education Department of Hainan Province (Project number: Hnky2020-19; Hnky2021-18), the Famous teachers' Studio of Hainan University (Grant No. hdms202022), the Natural Science Foundation of Hainan Province (Grant No. 119MS001), and the Educational and Teaching Reform Research Project of Hainan University (Grant No. hdjy2074; Grant No. hdjy2106).

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J.Z. wrote the paper. H.X. and C.L. performed the experiments. Y.W. analyzed the data, and D.L. and S.Z. conceived and designed the experiments. All authors read and approved the final manuscript.

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Correspondence to Debing Liu or Shenghe Zhao.

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Zhang, J., Xu, H., Li, C. et al. A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye. J Anal Sci Technol 12, 12 (2021). https://doi.org/10.1186/s40543-021-00262-w

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Keywords

  • Aptasensor
  • ATP detection
  • Molecular logic gate
  • Label-free fluorescence
  • Enzyme-free
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