Table 2 Validation parameters of the regression equations and assay results of the proposed chromatographic methods for the simultaneous quantitation of SBR and LPV

Linearity range

0.8–25.6 (μg band^− 1)

0.4–12.8 (μg band^− 1)

6.00–100.00 (μg ml^− 1)

4.0–80.0 (μg ml^− 1)

Slope (X² coefficient)

− 0.0223

− 0.0248

–

–

SE of Slope (X² coefficient)

0.0006

0.0007

–

–

Slope (X coefficient)

1.0976

0.6093

0.0454

0.1031

SE of slope (X coefficient)

0.0170

0.0094

0.0004

0.0008

Intercept

0.3402

0.5385

0.0283

− 0.0257

SE of intercept

0.0688

0.0191

0.0258

0.0353

Correlation coefficient (r)

0.9998

0.9998

0.9996

0.9998

LOD

0.21 (μg band⁻¹)

0.10 (μg band^− 1)

1.88 (μg ml^− 1)

1.13 (μg ml^− 1)

LOQ

0.63 (μg band^− 1)

0.31 (μg band^− 1)

5.69 (μg ml^− 1)

3.42 (μg ml^− 1)

Accuracy ^a

99.78 ± 0.970

100.04 ± 1.090

99.87 ± 1.270

99.64 ± 1.190

Precision ^b

Intra-day

0.575

0.862

0.613

1.276

Inter-day

1.274

1.543

1.628

1.903