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Table 2 Validation parameters of the regression equations and assay results of the proposed chromatographic methods for the simultaneous quantitation of SBR and LPV

From: Simultaneous chromatographic analysis of Sofosbuvir/Ledipasvir in their combined dosage form: an application to green analytical chemistry

Validation parametersHPTLC-densitometric methodRP HPLC-UV method
SBRLPVSBRLPV
Linearity range0.8–25.6 (μg band− 1)0.4–12.8 (μg band− 1)6.00–100.00 (μg ml− 1)4.0–80.0 (μg ml− 1)
Slope (X2 coefficient)− 0.0223− 0.0248
SE of Slope (X2 coefficient)0.00060.0007
Slope (X coefficient)1.09760.60930.04540.1031
SE of slope (X coefficient)0.01700.00940.00040.0008
Intercept0.34020.53850.0283− 0.0257
SE of intercept0.06880.01910.02580.0353
Correlation coefficient (r)0.99980.99980.99960.9998
LOD0.21 (μg band−1)0.10 (μg band− 1)1.88 (μg ml− 1)1.13 (μg ml− 1)
LOQ0.63 (μg band− 1)0.31 (μg band− 1)5.69 (μg ml− 1)3.42 (μg ml− 1)
Accuracy a99.78 ± 0.970100.04 ± 1.09099.87 ± 1.27099.64 ± 1.190
Precision b    
Intra-day0.5750.8620.6131.276
Inter-day1.2741.5431.6281.903
  1. aMean ± SD
  2. bRSD%, n = 9