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Table 1 Multiple reaction monitoring (MRM) transitions with optimized fragmentation voltage and collision energy

From: Stability study of opioids and benzodiazepines in urine samples by liquid chromatography tandem mass spectrometry

Compound RT (min) MW ISTD MRM transition ( m / z ) Fragmentor (V) CE (eV)
Opiates       
 6-Acetylmorphine 1.73 327.2 Oxycodone-d3 328.2 > 165.1 173 48
328.2 > 211.1 173 24
 Buprenorphine 3.27 467.3 Buprenorphine-d4 468.3 > 55.1 208 44
468.3 > 396.2 208 40
 Hydromorphone 0.92 285.2 Hydromorphone-d3 286.2 > 185.1 158 28
286.2 > 128.1 158 64
 Norbuprenorphine 2.70 413.3 Norbuprenorphine-d3 414.3 > 152.1 235 100
414.3 > 165.1 235 104
 Oxycodone 1.69 315.2 Oxycodone-d3 316.2 > 241.1 178 28
316.2 > 256.1 178 24
 Oxymorphone 0.75 301.1 Oxymorphone-d3 302.1 > 284.2 179 16
302.1 > 227.1 179 28
 Morphine 0.7 285.2 Morphine-d3 286.2 > 165.1 161 48
286.2 > 157.1 161 44
 Hydrocodone 1.74 299.2 Hydrocodone-d3 300.2 > 199.1 167 28
300.2 > 171.1 167 40
Benzodiazepines       
 Alpha hydroxy alprazolam 3.35 324.1 Alpha hydroxy alprazolam-d5 325.1 > 216.1 105 40
325.1 > 279.1 105 40
 Alprazolam 3.69 308.1 Alprazolam-d5 309.1 > 281.1 99 28
309.1 > 205.1 99 44
 Nordiazepam 3.72 270.1 Temazepam-d5 271.1 > 140.1 161 24
271.1 > 208.1 161 28
 Oxazepam 3.38 286.1 Oxazepam-d5 287.1 > 241.1 167 16
287.1 > 269.1 167 8
 Temazepam 3.73 300.1 Temazepam-d5 301.1 > 255.1 198 20
301.1 > 177.1 198 40
  1. Italicized transitions used for quantification. RT, retention time; MW, molecular weight; ISTD, internal standard; CE, collision energy.