- Research article
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Antimicrobial activities of silver nanoparticles synthesized from Lycopersicon esculentum extract
Journal of Analytical Science and Technology volume 5, Article number: 40 (2014)
It has been known for quite some time now that silver nanoparticles (AgNP) can inhibit microbial growth and even kill microbes. Our investigation reports the antimicrobial activity of AgNP against a model bacterium, Escherichia coli.
The aqueous extract of Lycopersicon esculentum (red tomato) was used for the rapid synthesis of AgNP, which is very simple and eco-friendly in nature. The UV-visible spectroscopy technique was employed to establish the formation of AgNP.
The transmission electron microscopic images showed that the particles were of mostly spherical shape. For the bacteriological tests, the microorganism E. coli was inoculated on Luria broth (LB) agar plate in the presence of varied amounts of AgNP. The antibacterial activity was obvious from the zone of inhibition. At concentration 20 μg/ml and above, the AgNP showed a clear zone of inhibition and the minimum inhibitory concentration of AgNP to E. coli was 50 μg/ml. Growth rates and bacterial concentrations were determined by measuring optical density at 600 nm at different time points.
From the slope of the bacterial growth curve, it has been concluded that the nanoparticles are bacteriostatic at low concentration and bactericidal at high concentration. So these nanoparticles are believed to act as preventive for bacterial contamination.
Disease-causing microbes that have become resistant to drug therapy are an increasing public health problem. Many researchers are now engaged in developing new effective antimicrobial reagents with the emergence and increase of microbial organisms resistant to multiple antibiotics, which will increase the cost of health care. Therefore, there is an urgent need to develop new bactericides. Silver has been used for years in the medical field for antimicrobial applications such as burn treatment (Parikh et al. ; Ulkur et al ), elimination of microorganisms on textile fabrics (Jeong et al. ; Lee et al. ; Yuranova et al. ), disinfection in water treatment (Russell and Hugo ; Chou et al. ), prevention of bacteria colonization on catheters (Samuel and Guggenbichler ; Alt et al. ; Rupp et al. ), etc. It has also been found to prevent HIV from binding to host cells (Sun et al. ), but the effects of silver nanoparticles (AgNP) on microorganisms have not been developed fully. Nanosilver, being less reactive than silver ions, is expected to be more suitable for medical applications. Reducing the particle size of metals is also an efficient and reliable tool for improving their biocompatibility, which facilitates their applications in different fields such as bioscience and medicine. The mechanism of the bacterial effect of AgNP as proposed is due to the attachment of AgNP to the surface of the cell membrane, thus disrupting permeability and respiration functions of the cell (Kevitec et al. ). It is also proposed that AgNP not only interact with the surface of a membrane but can also penetrate inside the bacteria (Morones et al. ). The antibacterial activity of AgNP is significantly enhanced when it is modified with sodium dodecyl sulfate (SDS) (Kevitec et al. 2005; Carpenter ).
In this study, we have investigated the antimicrobial effects of silver nanoparticles prepared by a biosynthesis method. The chemical reduction method is widely used to synthesize AgNP because AgNP could be synthesized under a mild as well as on a large scale (Cao et al. ). However, the use of environmentally benign materials like plant leaf extract, bacteria, and fungi for the synthesis of silver nanoparticles is more acceptable as they offer several benefits over chemical methods like conditions of high temperature, pressure, and toxic chemicals which are not required in the synthesis protocol (Singh et al. ). Therefore, preparation of AgNP by a green synthesis approach has compatibility for pharmaceutical and biomedical applications.
In the present work, the synthesis of silver nanoparticles has been carried out using the aqueous extract of Lycopersicon esculentum (red tomato). The water extract of tomato juice mostly contains proteins and water-soluble organic acids (Gould ) which are believed to act as stabilizing and reducing agents, respectively. With these nanoparticles, a preliminary test for antibacterial activity was carried out by cup diffusion method and the effects of AgNP on bacterial growth has been studied by employing minimum inhibitory concentration (MIC) method. Results obtained by us prove that AgNP prepared by the green method is suitable for the formulation of new types of bactericidal materials.
Results and discussion
Characterization and optimization of AgNP preparation
The absorbance spectra of the AgNP were analyzed by using a ‘SHIMADZU’ UV 1800 spectrophotometer (Shimadzu Corporation, Kyoto, Japan). AgNP exhibited a reddish yellow color in water due to the excitation of the localized surface plasmon vibrations of the metal nanoparticles. Generally, the surface plasmon resonance (SPR) bands are influenced by the size, shape, morphology, composition, and dielectric environment of the synthesized nanoparticles (Kelly et al. ; Stepanov ). Previous studies showed that spherical AgNP contribute to the absorption bands at around 400 nm in the UV-visible spectra (Maiti et al. ; Barman et al. ). The SPR band due to AgNP was observed in our case at around 410 nm (Figure 1) when 3 × 10−3 M silver nitrate solution was used. This strongly suggested that AgNP were nearly spherical in shape and it was confirmed by the transmission electron microscopy (TEM) results.
In this study, AgNP have been synthesized both in the presence and in the absence of a stabilizer and both anionic and neutral surfactants were used one at a time. Though soluble proteins and amino acids present in L. esculentum extract were expected to act as stabilizer for nanoparticles (Barman et al. ), a smooth and narrow absorption band of AgNP at 410 nm was observed only in the presence of SDS of 3 × 10−3 M (Figure 1A). So we preferred to synthesize AgNP using SDS as the stabilizing agent.
An absorption band was observed at 410 nm for 1:1 extract composition. The plasmon band shifted to higher values with the increase of the concentrations of tomato in aqueous extracts and reached to 415 nm for 3:2 composition (Figure 1B). At concentrations higher than 3:2 composition, the plasmon band shifted to higher values and the extinction coefficient of the band decreased appreciably. However, tomato extract of 1:1 composition was used throughout the work.
A bathochromic shift of the SPR bands from 388 to 445 nm was observed while the concentration of AgNO3 varied from 3 × 10−3 to 5 × 10−2 M keeping the extract composition constant at 1:1 using SDS of 3 × 10−3 M (Figure 1C). When the particle size increased, the absorption peak shifted towards the red wavelength, which indicated the formation of larger sized nanoparticles (Peng et al. ).
The shape and size distribution of the synthesized AgNP were characterized by TEM study. The TEM images were taken using JEOL JEM-2100 high-resolution transmission electron microscope (HR-TEM; JEOL Ltd., Akishima-shi, Japan). Samples for the TEM studies were prepared by placing a drop of the aqueous suspension of particles on carbon-coated copper grids followed by solvent evaporation under vacuum. The TEM images of AgNP produced from 1:1 composition of tomato extract showed that the particles were mostly spherical and their sizes varied from 10 to 40 nm. Selected area electron diffraction (SAED) pattern illustrated the crystalline nature of AgNP (Figure 2).
Antibacterial activity of AgNP against the microorganism
Preliminary test for antibacterial activity
The antimicrobial activity of AgNP was evaluated against Escherichia coli by cup diffusion method. Approximately 106 colony-forming units (CFU) of the microorganism E. coli were inoculated on Luria broth (LB) agar plate, and then different concentrations of AgNP (1, 2, 5, 10, 20, 50, 100, and 200 μg/ml) were added to the well present in the LB agar plate. A reaction mixture containing no AgNP was put in the well in the LB plate and cultured under the same condition as the control test. All the LB plates were incubated at 37°C overnight. After incubation, the plates were observed for the presence of a zone of inhibition. The antibacterial activity of AgNP was proved from the zone of inhibition (Figure 3). At concentration 20 μg/ml and above, the AgNP showed a clear zone of inhibition. No zone of inhibition was found in the vehicle control well (spot in the middle of the plate) which suggested that the antimicrobial activity was specifically due to AgNP.
Evaluation of antibacterial effectiveness using minimum inhibitory concentration method
The antimicrobial activity of AgNP was evaluated using the MIC method. The antimicrobial effectiveness was determined against the bacterial concentration of 106 CFU/ml with different concentrations of AgNP (0.2, 0.5, 1, 2, 5, 10, 20, 50, and 100 μg/ml). The cultures were incubated at 37°C at 250 rpm. Bacterial concentrations were determined by measuring optical density (OD) at 600 nm (0.1 OD600 corresponding to 108 cells per milliliter). With the increase of concentration of nanoparticles, the final bacterial concentration decreased. When the concentration of AgNP was 50 μg/ml, growth of E. coli was completely inhibited, which indicated that the MIC of AgNP to E. coli was 50 μg/ml (Figure 4).
Effect of AgNP on bacterial growth
To determine the growth curve in the presence of silver nanoparticles, E. coli bacteria were grown in liquid LB medium till they reached the log phase. Then they were diluted in fresh LB liquid medium to optical density (OD600) 0.05, 0.1, and 0.2. AgNP solution was added into the cell culture medium at different concentrations, and the culture was incubated at 37°C and 250 rpm. Growth rates and bacterial concentrations were determined by measuring OD at 600 nm at different time points (Figure 5A,B,C).
The slope of the bacterial growth curve continuously decreased with increasing nanoparticle concentration. This means that at low concentration of nanoparticles, the growth of bacteria was delayed and at higher concentration, growth was completely inhibited. So it can be concluded that the nanoparticles are bacteriostatic at low concentration and bactericidal at high concentration. It was also clear from the graphs that the bacterial growth was dependent on the initial number of cells present in the medium. It was observed that at lower initial OD, the AgNP concentration necessary to completely inhibit bacterial growth was also low. So silver nanoparticles produced by us will be suitable for preventing bacterial contamination.
Chemical antimicrobial agents are increasingly becoming resistant to a wide spectrum of antibiotics. An alternative way to overcome the drug resistance of various microorganisms is therefore urgently needed. Ag ions and silver salts have been used for decades (Silver and Phung ) as antimicrobial agents in various fields due to their growth-inhibitory abilities against microorganisms. However, there are some limitations in using Ag ions or Ag salts as antimicrobial agents. Probable reasons include the interfering effects of salts. This type of limitation can be removed by using silver in nano form. Due to the increase of the surface area in nano state, the contact area between Ag(0) and that of the microorganism increases. To use AgNP against microbes in various fields, it is important and necessary to prepare AgNP in a green environment. In this study, we report a green method for the preparation of AgNP which is environmentally benign and cost-effective.
For the assessment of the antimicrobial effects of AgNP, E. coli was used in our study. The effect was investigated by growing E. coli on agar plates and in liquid LB medium, supplemented with AgNP. The bacterial growth was completely inhibited in the presence of AgNP on the LB agar plate. The inhibition solely depended upon the AgNP concentration. It showed a clear zone of inhibition at and above the concentration 20 μg/ml.
To study the antimicrobial effectiveness of AgNP, we treated a bacterial concentration at high CFU (106/ml) with varying concentrations of AgNP from 0.2 to 100 μg/ml. When the concentration of AgNP was increased, the bacterial concentration was found to decrease. At concentration 50 μg/ml of AgNP, the growth of E. coli was completely inhibited, which indicated that the minimum inhibitory concentration was 50 μg/ml (Figure 4). Since high CFU are seldom found in real-life systems, it may be concluded that these AgNP have a biocidal effect and effectiveness in delaying bacterial growth, findings which may lead to valuable inventions in the future in various fields like in antimicrobial systems as well as medical devices.
The slope of the bacterial growth curves (Figure 5A,B,C) continuously decreased with increasing nanoparticle concentration. This indicated that at low concentration of nanoparticles, bacterial growth was delayed and growth was completely inhibited at higher concentrations. So it appears that these particles are bacteriostatic at low concentration and bactericidal at high concentration. It is also clear from the graphs that the bacterial growth is dependent on the initial number of cells present in the medium. It was observed that at lower initial OD, the AgNP concentration necessary to completely inhibit bacterial growth was also low. So it is confirmed that these nanoparticles may be used to prevent bacterial contamination.
The mechanism of the inhibitory effects of Ag ions on microorganisms is partially known. It is reported that the positive charge on the silver ion is the reason for antimicrobial activity as it can attract the negatively charged cell membrane of microorganisms through the electrostatic interaction (Dibrov et al. ; Hamouda et al. ). Due to their unique size and greater surface area, silver nanoparticles can easily reach the nuclear content of bacteria (Chen et al. ; Chudasama et al. ). A survey of the literature showed that the electrostatic attraction between negatively charged bacterial cells and positively charged nanoparticles was crucial for the antibacterial activity (Stoimenov et al. ). The AgNP used in this study, however, received negative charge from SDS, an anionic surfactant, used during synthesis. The bacterium E. coli being gram-negative, the interaction with the negatively charged nanoparticles might have occurred through ‘pit’ formation in the cell wall of the bacteria (Sondi and Salopek-Sondi ) which helped the permeability and resulted in cell death.
Silver nitrate and SDS, both of AR grade, were purchased from Sigma-Aldrich Chemical Ltd. (St Louis, MO, USA) Sodium hydroxide was purchased from Merck (Darmstadt, Germany). Double-distilled de-ionized water was used in all experiments.
Green synthesis of silver nanoparticles by L. esculentum extract
Silver nanoparticles were made according to the recipe described below. For this purpose, red tomato (L. esculentum) was collected from the local market and washed with double-distilled de-ionized water. The tomato skin was removed and the whole mass was squeezed to get tomato juice. Then it was diluted two times and filtered using a Whatman filter paper to get the aqueous extract of the red tomato.
AgNP were produced by reduction of silver nitrate solution by using red tomato extract. Ten milliliters of aqueous red tomato extract was mixed with 10 ml of 3 × 10−3 M SDS solution and cooled in ice-cold water for few minutes. The solution was made alkaline (pH 9) with 0.15 N sodium hydroxide solution. After that, 8 ml of 3 × 10−3 M aqueous silver nitrate was added into it dropwise with continuous stirring. The mixture was then heated for 20 min at 80°C. The color of the solution gradually changed from colorless to reddish yellow. The reddish yellow color indicated the formation of AgNP.
We have described a simple and green method for the synthesis of AgNP by using the aqueous extract of red tomato. The formation of AgNP was confirmed by UV-visible spectroscopy. The TEM images showed that the particles were mostly spherical. These biosynthesized AgNP were then used to demonstrate antimicrobial activity against a model bacterium, E. coli. The antibacterial activity of AgNP was apparent from the zone of inhibition. At concentrations 20 μg/ml and above, the AgNP showed a clear zone of inhibition and the MIC of AgNP to E. coli was 50 μg/ml. Growth rates and bacterial concentrations were determined by measuring OD at 600 nm at different time points. From the slope of the bacterial growth curve, it has been concluded that the nanoparticles are bacteriostatic at low concentration and bactericidal at high concentration. So these nanoparticles are believed to act as preventive for bacterial contamination.
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We are thankful to Central Research Facility at IIT Kharagpur, India, for the HR-TEM measurements.
The authors declare that they have no competing interests.
SM and DK carried out the experiments. SM, GB, and DK drafted the manuscript. JKL and SKG guided the research and modified the manuscript. All authors read and approved the final manuscript.
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Maiti, S., Krishnan, D., Barman, G. et al. Antimicrobial activities of silver nanoparticles synthesized from Lycopersicon esculentum extract. J Anal Sci Technol 5, 40 (2014). https://doi.org/10.1186/s40543-014-0040-3
- Silver nanoparticle
- Green synthesis
- Lycopersicon esculentum
- Antibacterial activity
- Escherichia coli