Fig. 7

Online HIC-MS of mAb mixtures on a maXis II Q-TOF mass spectrometer. a Total ion chromatogram demonstrating the separation of mAb1 and mAb2. The dashed line indicates the gradient changes of mobile phase B. b Mass spectrum of mAb2 showing the detection of monomers, dimers (30 × zoom-in), and trimers (100 × zoom-in). c Deconvoluted mass spectrum of mAb2 monomer with annotated glycosylation forms (red triangle, fucose; blue square, GlcNAc; green circle, mannose; yellow circle, galactose); hollow square represents the loss of one GlcNAc (− 203 Da), hollow triangle represents the preservation of C-terminal Lys on heavy chain (+ 128 Da), and an asterisk represents the addition of a hexose (+ 162 Da). GxF indicates Fc-oligosaccharides terminated by x number of galactoses. Reprinted with permission from Ref (Chen et al. 2018a). Copyright 2017 American Chemical Society