Fig. 17

Denatured and native mass analysis of brentuximab vedotin. a ESI mass spectra of deglycosylated brentuximab vedotin in classical denatured conditions. ESI mass spectra of brentuximab vedotin under native conditions after deglycosylation on b QTOF; c orbitrap and with glycosylation on d QTOF and e orbitrap. Left section shows the full scan spectra on the mass range of 1000–8000; the middle section shows the spectra with mass range of 6000–6250 and 6200–6500 for deglycosylated and glycosylated brentuximab vedotin, respectively; the right section shows the deconvoluted spectra for drug load profiles. Reprinted with permission from Ref (Debaene et al. 2014). Copyright 2014 American Chemical Society