Fig. 11

RP HPLC separates IgG2 disulfide isoforms: 4 g injected. a UV absorbance of reversed-phase chromatogram at 214 nm. b Total ion chromatogram with MS scans (peaks) for mass measurement and CID scans (valleys) for top-down analysis. c MagTran was used to deconvolute ESI mass spectra of intact monoclonal IgG2 antibody reversed-phase peaks 1–4. Reprinted with permission from Ref (Bondarenko et al. 2009). Copyright 2009 American Chemical Society