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Journal of Analytical Science and Technology
Fig. 2 | Journal of Analytical Science and Technology

Fig. 2

From: Direct and accurate miRNA detection based on CRISPR/Cas13a-triggered exonuclease-iii-assisted colorimetric assay

Fig. 2

Feasibility of the approach. A Schematic illustration of the fluorescent assay to test the trans-cleavage activity of CRISPR-Cas13a system. B Fluorescence intensity of the FAM labeled H1 probe during the trans-cleavage process of Cas13a-crRNA13a system. a, H1 probe (before assembly); b, H1 probe; c, H1 probe + miRNA-155; d, H1 probe + miRNA-155 + Cas13a-crRNA13a; e, H1 probe + miRNA-155 + Cas13a-crRNA13a + H2 probe. C Schematic illustration of the fluorescent assay to test the selectivity of CRISPR-Cas13a system. D Fluorescence intensity of the FAM labeled H1 probe when Cas13a-crRNA13a system was incubated with miRNA-155 and non-specific miRNA. a, reporter probe (FAM and BHQ were labeled on both terminus of reporter probe); b, reporter probe + Cas13a-crRNA13a; c, reporter probe + Cas13a-crRNA13a + miRNA-155; d, reporter probe + Cas13a-crRNA13a + non-specific miRNA. E Absorbance of the approach under different experimental conditions

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