Table 2 The experimental design and results for robustness study
Exp. No | FA % in MP(A) | FA % in MP(B) | Organic phase % | Temp. (°C) | Flow rate (ml/min) | Peak ratio (n = 3) |
|---|---|---|---|---|---|---|
1 | − 1 | 1 | 1 | − 1 | 1 | 2.15 ± 0.01 |
2 | − 1 | − 1 | 1 | 1 | 1 | 1.92 ± 0.03 |
3 | 1 | − 1 | 1 | 1 | − 1 | 2.39 ± 0.01 |
4 | 1 | 1 | − 1 | 1 | − 1 | 2.15 ± 0.01 |
5 | 1 | − 1 | − 1 | − 1 | 1 | 2.51 ± 0.01 |
6 | − 1 | 1 | 1 | 1 | − 1 | 2.14 ± 0.01 |
7 | − 1 | − 1 | − 1 | − 1 | − 1 | 2.14 ± 0.02 |
8 | 1 | 1 | − 1 | 1 | 1 | 2.29 ± 0.01 |
9 | − 1 | 1 | − 1 | − 1 | − 1 | 2.13 ± 0.01 |
10 | 1 | − 1 | − 1 | 1 | 1 | 2.53 ± 0.01 |
11 | 1 | − 1 | 1 | − 1 | − 1 | 2.48 ± 0.01 |
12 | 1 | 1 | 1 | − 1 | 1 | 2.37 ± 0.02 |
13 | 0 | 0 | 0 | 0 | 0 | 1.84 ± 0.01 |
p values | 0.344 | 0.538 | 0.985 | 0.346 | 0.958 |