Table 3 Matrix effects for VEN and IS in plasma samples of the rat and dog
Matrix factor1 | IS-normalized | ||
|---|---|---|---|
VEN | IS | Matrix effect2 | |
Rat plasma | |||
LQC (3 ng/mL) | |||
A | 0.77 | 0.83 | 0.92 |
B | 1.06 | 1.00 | 1.06 |
C | 0.97 | 1.03 | 0.95 |
D | 0.99 | 1.07 | 0.92 |
E | 0.99 | 0.99 | 1.00 |
F | 0.91 | 0.98 | 0.93 |
A (Hemolyzed) | 1.00 | 1.02 | 0.97 |
B (Hemolyzed) | 1.05 | 0.99 | 1.06 |
Mean ± SD | 0.97 ± 0.09 | 0.99 ± 0.07 | 0.98 ± 0.06 |
CV (%)3 | 9.5 | 7.0 | 5.8 |
HQC (800 ng/mL) | |||
A | 0.96 | 0.94 | 1.02 |
B | 1.00 | 1.00 | 1.00 |
C | 1.01 | 1.00 | 1.01 |
D | 1.00 | 0.96 | 1.03 |
E | 0.98 | 0.99 | 0.99 |
F | 1.04 | 1.00 | 1.04 |
A (Hemolyzed) | 1.01 | 1.02 | 0.99 |
B (Hemolyzed) | 1.03 | 1.01 | 1.02 |
Mean ± SD | 1.00 ± 0.02 | 0.99 ± 0.03 | 1.01 ± 0.02 |
CV (%) | 2.5 | 2.7 | 2.0 |
Dog plasma | |||
LQC (3 ng/mL) | |||
A | 0.82 | 0.79 | 1.05 |
B | 0.96 | 0.97 | 0.99 |
C | 0.88 | 0.93 | 0.94 |
D | 1.14 | 0.92 | 1.24 |
E | 1.09 | 0.98 | 1.11 |
F | 0.93 | 0.90 | 1.04 |
A (Hemolyzed) | 0.80 | 0.80 | 0.99 |
B (Hemolyzed) | 0.97 | 0.96 | 1.01 |
Mean ± SD | 0.95 ± 0.12 | 0.91 ± 0.07 | 1.05 ± 0.09 |
CV (%) | 12.8 | 8.3 | 8.8 |
HQC (800 ng/mL) | |||
A | 1.03 | 0.93 | 1.11 |
B | 1.03 | 0.97 | 1.06 |
C | 1.06 | 1.01 | 1.05 |
D | 1.11 | 1.03 | 1.08 |
E | 1.07 | 1.02 | 1.05 |
F | 1.04 | 1.00 | 1.04 |
A (Hemolyzed) | 1.03 | 1.01 | 1.05 |
B (Hemolyzed) | 1.04 | 1.01 | 1.03 |
Mean ± SD | 1.05 ± 0.03 | 1.00 ± 0.03 | 1.05 ± 0.03 |
CV (%) | 2.7 | 3.3 | 2.6 |