Skip to main content

Table 1 Information of calibration standard and isotope labeled materials applied in the current study

From: Development of a certified reference material for the analysis of vitamins in multivitamin tablets

Analyte

Calibration standard material

Purity (%)a

Isotope labeled materialb

Thiamine

Thiamine HCl (Dr. Ehrenstofer GmbH)

95.1 ± 0.1

13C4-Thiamine HCl (IsoSciences LLC.)

Riboflavin

Riboflavin (Dr. Ehrenstofer GmbH)

99.8 ± 0.1

13C4, 15N2-Riboflavin (IsoSciences LLC.)

Nicotinamide

Nicotinamide (Chromadex)

99.4 ± 0.3

Nicotindamide-d4 (CDN Isotope)

Pantothenic acid

Calcium Pantothenate (Chromadex)

99.6 ± 0.2

13C3, 15N1-Calcium Pantothenate (Cambridge Isotope Laboratories)

Pyridoxine

Pyridoxine HCl (Chromadex)

99.5 ± 0.7

13C4-Pyridxoen HCl (Cambridge Isotope Laboratories)

Biotin

Biotin (Dr. Ehrenstofer GmbH)

99.7 ± 0.1

Biotin- d2 (IsoSciences LLC.)

Folic acid

Folic acid (Chromadex)

89.2 ± 0.1

13C5-Folic acid (Merck Eprova AG)

Retinol

Retinol (Sigma-Aldrich)

 ≥ 95

Tocopherols

α-Tocopherol (Sigma-Aldrich)

 ≥ 95

Cholecalciferol

Cholecalciferol (Sigma-Aldrich)

 ≥ 95

Phylloquinone

Phylloquinone (Sigma-Aldrich)

 ≥ 95

  1. aPurity assay was performed by mass balance method for 7 WSVs including thiamine HCl, riboflavin, nicotinamide, calcium pantothenate, pyridoxine HCl, biotin and folic acid. Purities of retinol, α-tocopherol, cholecalciferol and phylloquinone were based on the manufacturer’s certificates
  2. bIsotope labeled materials were utilized an internal standard for IDMS method for certification of 7 WSVs