A short novel antimicrobial peptide BP100-W with antimicrobial, antibiofilm and anti-inflammatory activities designed by replacement with tryptophan

Journal of Analytical Science and Technology

Table 3 Fractional inhibitory concentration index (FICI)^a of peptides in combination with conventional antibiotics against multidrug-resistant Pseudomonas aeruginosa (MDRPA) (CCARM 2095)

Combinations		MIC_A (μM)	[A] (μM)	FIC_A	MIC_B (μM)	[B] (μM)	FIC_B	FICI	Combination effect
A: Antibiotics	B: Peptides	MIC_A (μM)	[A] (μM)	FIC_A	MIC_B (μM)	[B] (μM)	FIC_B	FICI	Combination effect
Chloramphenicol	BP100	1024	128	0.125	16	4	0.25	0.375	Synergy
Chloramphenicol	BP100-W	1024	256	0.25	16	4	0.25	0.5	Synergy
Ciprofloxacin	BP100	1024	128	0.125	16	4	0.25	0.375	Synergy
Ciprofloxacin	BP100-W	1024	64	0.0625	16	4	0.25	0.3125	Synergy
Oxacillin	BP100	1024	256	0.25	16	8	0.5	0.75	Additive
Oxacillin	BP100-W	1024	128	0.125	16	4	0.25	0.375	Synergy

MIC_A: MIC of antibiotic A alone, [A]: MIC of antibiotic A in combination. FIC_A = [A]/MIC_A
MIC_B: MIC of peptide B alone, [B]: MIC of peptide B in combination. FIC_B = [B]/MIC_B
^aFICI = FIC_A + FIC_B. FICI ≤ 0.5 is synergy, 0.5 < FICI ≤ 1.0 is additive, 1.0 < FICI ≤ 4.0 is indifferent