Table. 1 Method validation and enrichment effect of the developed method
Compounds | Liner range (µg/mL) | R2 | LOD (ng/mL) | Recovery (%, n = 5) | Precision (%) | Standards EF | U251 cell | |||
|---|---|---|---|---|---|---|---|---|---|---|
1.5 µg/mL | 0.75 µg/mL | 0.3 µg/mL | Intra-day (n = 5) | Inter-day (n = 5,D = 3) | Concentration (µg/mL) | |||||
SAH | 0.025–5 | 0.998 | 5.2 | 96.9 | 89.4 | 93.7 | 10.7 | 10.4 | 32.4 | – |
ACC | 0.0025–2.5 | 0.997 | 1.4 | 76.9 | 104.3 | 103.2 | 3.4 | 5.6 | 20.6 | – |
Ser | 0.05–12.5 | 0.984 | 25.0 | 104.5 | 95.3 | 113.3 | 4.7 | 8.6 | 22.0 | 0.2 |
Cystathionine | 0.0125–1.25 | 0.998 | 11.7 | 96.3 | 89.8 | 97.3 | 3.6 | 10.0 | 26.0 | 0.02 |
Val | 0.03–3 | 1.000 | 7.5 | 107.7 | 103.8 | 114.5 | 1.8 | 4.3 | 26.9 | 0.1 |
Asn | 0.03–3 | 0.996 | 7.5 | 103.7 | 94.7 | 111.1 | 2.4 | 8.6 | 19.5 | 0.04 |
Glu | 0.0125–12.5 | 0.998 | 6.2 | 101.5 | 93.6 | 111.1 | 5.0 | 6.4 | 25.6 | 0.1 |
Cystine | 0.025–12.5 | 0.991 | 15.1 | 100.2 | 97.2 | 93.0 | 3.6 | 8.0 | 24.3 | – |
Cys | 0.0125–5 | 0.999 | 5.0 | 99.4 | 93.7 | 95.7 | 3.5 | 6.1 | 33.1 | 0.1 |
Asp | 0.025–12.5 | 0.993 | 6.9 | 109.0 | 94.8 | 103.5 | 6.1 | 12.4 | 24.4 | – |
Met | 0.0125–5 | 0.991 | 5.0 | 99.2 | 95.7 | 96.7 | 2.8 | 5.3 | 27.8 | 0.1 |
GSH | 0.0125–2.5 | 0.998 | 7.0 | 96.8 | 94.1 | 104.9 | 5.2 | 18.8 | 18.1 | 0.04 |
S-Carboxymethylcysteine | 0.025–12.5 | 0.993 | 17.5 | 100.2 | 89.0 | 98.9 | 5.4 | 14.3 | 14.4 | – |
S-Lactoylglutathione | 0.0125–5 | 0.999 | 1.7 | 98.3 | 95.3 | 101.1 | 5.8 | 16.2 | 17.5 | – |
Gama-Glu-Cys | 0.25–12.5 | 0.994 | 203.9 | 99.2 | 91.7 | 105.2 | 7.1 | 6.8 | 18.1 | – |
Homo-cys | 0.0125–5 | 0.999 | 8.3 | 101.2 | 93.9 | 88.1 | 2.8 | 5.7 | 25.0 | – |