Skip to main content

Table 3 Intra-day and inter-day precision of derivative ratio, delta absorbance, first derivative of delta A for the determination of SF in presence of VP and for spectrophotometric (UV) and spectrofluorimetric (flu) determination of VP in presence of SF using NBD-Cl and MBTH method

From: Eco-friendly analytical methods for the determination of compounds with disparate spectral overlapping: application to antiviral formulation of sofosbuvir and velpatasvir

Determination of SF in presence of VP
SF (μg/mL) Mean % recovery ± SD RSD% (a) Er (%) (b)
2DD ΔA ΔD1 2DD Δ A Δ D1 2DD ΔA ΔD1
Intra-day precision
10 100.56 ± 0.64 99.14 ± 1.16 99.74 ± 1.817 0.64 1.17 1.82 0.56 −0.86 −0.26
30 101.64 ± 0.94 101.21 ± 1.52 101.98 ± 0.958 0.92 1.51 0.94 1.64 1.21 1.98
60 102.11 ± 0.19 101.18 ± 1.86 101.59 ± 1.36 0.18 1.84 1.34 2.11 1.18 1.59
Inter-day precision
10 100.37 ± 1.83 99.68 ± 0.58 100.77 ± 1.34 1.82 0.58 1.33 0.37 −0.31 0.77
30 101.29 ± 0.82 100.77 ± 1.02 101.55 ± 1.34 0.8 1.01 1.32 1.29 0.77 1.32
60 102.52 ± 0.44 101.02 ± 0.48 101.16 ± 1.02 0.42 0.47 1.01 2.52 1.02 1.16
Determination of VP in presence of SF
VP (μg/mL) Mean % recovery ± SD RSD %(a) Er (%)(b)
NBD-Cl MBTH NBD-Cl MBTH NBD-Cl MBTH
UV Flu UV UV Flu UV UV Flu UV
Intra-day precision
20 μg/ml (UV)
4 μg/ml (flu)
99 ± 1 100.28 ± 1.64 100.33 ± 1.91 1.01 1.63 1.9 −1 0.28 0.33
50 μg/ml (UV)
10 μg/ml (flu)
99.2 ± 1.113 98.98 ± 0.82 100.97 ± 1.04 1.122 0.83 1.03 −0.8 −1.01 0.97
150 μg/ml (UV)
15 μg/ml (flu)
100.66 ± 1.75 99.37 ± 0.66 100.32 ± 0.75 1.74 0.67 0.75 0.66 −0.62 0.32
Inter-day precision
20 μg/ml (UV)
4 μg/ml (flu)
99.88 ± 1.495 101.19 ± 1.56 99.08 ± 1.91 1.497 1.54 1.93 −0.11 1.19 −0.92
50 μg/ml (UV)
10 μg/ml (flu)
99.51 ± 0.922 99.54 ± 0.94 99.80 ± 1.00 0.927 0.95 1 −0.488 −0.46 −0.2
150 μg/ml (UV)
15 μg/ml (flu)
100.44 ± 1.236 99.17 ± 0.48 99.66 ± 0.79 1.23 0.49 0.79 0.44 −0.83 −0.34
  1. (a)% Relative standard deviation
  2. (b)% Error
\