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Table 5 Summary of validation parameter of SIAM of AV

From: Comprehensive stability-indicating method development of Avanafil Phosphodiesterase type 5 inhibitor using advanced Quality-by-Design approach

Parameter

AV

Detection wavelength (nm)

239

Linearity (μg/mL)

10–70

Regression coefficient (R2)

0.997

Regression equation

y = 1E + 06x − 3E + 06

LOD (μg/mL)

0.360

LOQ (μg/mL)

1.150

Intraday precision % RSD (n = 3)

0.326–0.697

Interday precision % RSD (n = 3)

1.680–1.800

% Assay ± RSD

(99.430–102.040) ± 0.997

% Recovery ± RSD

99.280 ± 0.239 to 101.420 ± 0.092