Table 2 Stability and matrix effect of HIST, His, and 5 BAs in spiked gastric fluid sample
Analytes | Conc. (ng/mL) | Stability* (%) | Matrix effect* (%) | ||||||
|---|---|---|---|---|---|---|---|---|---|
Room temperature | 4 °C | 3 freeze-thaw cycle | |||||||
24 h | 48 h | 72 h | 24 h | 48 h | 72 h | ||||
His | 250 | 86.38 ± 5.42 | 75.61 ± 7.63 | 74.06 ± 3.65 | 84.00 ± 4.99 | 81.05 ± 6.71 | 82.21 ± 2.35 | 96.32 ± 4.68 | 96.60 ± 5.68 |
500 | 78.13 ± 0.84 | 77.19 ± 3.86 | 77.71 ± 4.97 | 80.20 ± 5.18 | 77.55 ± 4.29 | 84.71 ± 4.32 | 91.82 ± 3.69 | ||
2500 | 75.09 ± 2.11 | 82.36 ± 3.69 | 81.97 ± 3.36 | 85.96 ± 5.79 | 85.92 ± 4.53 | 87.85 ± 6.08 | 97.57 ± 4.11 | ||
HIST | 25 | 89.07 ± 4.09 | 83.60 ± 6.05 | 87.31 ± 3.25 | 76.98 ± 6.24 | 86.51 ± 2.78 | 82.79 ± 4.26 | 97.49 ± 1.87 | 110.62 ± 9.98 |
50 | 76.82 ± 2.91 | 76.13 ± 2.49 | 80.55 ± 3.72 | 75.79 ± 6.13 | 82.68 ± 1.76 | 82.40 ± 4.00 | 89.14 ± 5.43 | ||
250 | 79.60 ± 4.57 | 87.67 ± 2.69 | 89.50 ± 1.52 | 83.82 ± 3.11 | 91.10 ± 1.07 | 90.02 ± 1.78 | 98.31 ± 2.39 | ||
UDCA | 25 | 94.89 ± 3.47 | 87.33 ± 5.82 | 88.31 ± 1.72 | 89.91 ± 3.04 | 96.95 ± 3.44 | 88.43 ± 4.11 | 99.22 ± 5.23 | 93.50 ± 7.52 |
50 | 82.35 ± 1.91 | 84.52 ± 2.92 | 84.54 ± 2.45 | 92.50 ± 3.34 | 91.04 ± 1.72 | 87.98 ± 1.78 | 84.03 ± 8.11 | ||
250 | 86.26 ± 1.90 | 85.97 ± 7.65 | 91.48 ± 1.27 | 93.68 ± 2.55 | 97.69 ± 2.33 | 91.94 ± 2.01 | 87.18 ± 5.47 | ||
CA | 25 | 96.08 ± 5.76 | 85.92 ± 4.79 | 87.62 ± 4.87 | 88.88 ± 4.44 | 90.85 ± 4.44 | 88.05 ± 2.63 | 98.29 ± 5.47 | 113.92 ± 11.87 |
50 | 83.21 ± 4.03 | 86.54 ± 5.39 | 88.81 ± 0.78 | 95.93 ± 6.86 | 88.62 ± 3.82 | 87.83 ± 4.07 | 88.17 ± 5.68 | ||
250 | 84.75 ± 1.06 | 86.20 ± 4.46 | 91.26 ± 2.32 | 91.91 ± 2.36 | 91.77 ± 2.00 | 89.53 ± 2.54 | 95.27 ± 3.48 | ||
CDCA | 25 | 95.78 ± 3.83 | 84.95 ± 3.26 | 91.83 ± 3.14 | 91.36 ± 3.29 | 101.14 ± 4.88 | 84.76 ± 5.28 | 75.61 ± 7.91 | 104.82 ± 10.00 |
50 | 87.57 ± 3.66 | 95.49 ± 5.48 | 92.60 ± 7.03 | 104.88 ± 2.04 | 94.61 ± 5.82 | 91.13 ± 3.96 | 70.77 ± 10.45 | ||
250 | 94.50 ± 2.36 | 91.40 ± 9.45 | 103.28 ± 2.14 | 108.03 ± 1.95 | 107.31 ± 1.46 | 100.74 ± 5.11 | 70.42 ± 6.12 | ||
DCA | 25 | 97.79 ± 5.06 | 92.93 ± 1.43 | 96.25 ± 6.98 | 96.85 ± 5.17 | 103.98 ± 4.02 | 91.67 ± 3.48 | 85.75 ± 4.44 | 104.15 ± 10.44 |
50 | 90.06 ± 1.98 | 97.35 ± 3.94 | 95.48 ± 4.81 | 107.31 ± 3.66 | 95.47 ± 5.17 | 91.59 ± 2.95 | 74.61 ± 6.28 | ||
250 | 91.50 ± 3.48 | 97.22 ± 9.01 | 98.24 ± 1.16 | 105.35 ± 1.59 | 106.21 ± 2.83 | 95.09 ± 4.32 | 72.67 ± 6.78 | ||
LCA | 25 | 98.08 ± 3.26 | 103.03 ± 3.45 | 104.43 ± 2.37 | 107.03 ± 1.78 | 105.87 ± 3.71 | 104.61 ± 3.06 | 87.37 ± 1.46 | 103.47 ± 10.18 |
50 | 93.41 ± 5.14 | 104.34 ± 1.18 | 104.08 ± 2.49 | 107.77 ± 3.97 | 107.37 ± 3.10 | 100.29 ± 4.77 | 76.51 ± 6.28 | ||
250 | 104.65 ± 3.50 | 101.39 ± 5.81 | 105.73 ± 7.50 | 99.73 ± 0.95 | 109.64 ± 3.94 | 97.68 ± 4.40 | 70.83 ± 9.21 | ||