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Table 2 Stability and matrix effect of HIST, His, and 5 BAs in spiked gastric fluid sample

From: UHPLC-MS/MS profiling of histidine and bile acid metabolism in human gastric fluid for diagnosis of gastric diseases

Analytes Conc. (ng/mL) Stability* (%) Matrix effect* (%)
Room temperature 4 °C 3 freeze-thaw cycle
24 h 48 h 72 h 24 h 48 h 72 h
His 250 86.38 ± 5.42 75.61 ± 7.63 74.06 ± 3.65 84.00 ± 4.99 81.05 ± 6.71 82.21 ± 2.35 96.32 ± 4.68 96.60 ± 5.68
500 78.13 ± 0.84 77.19 ± 3.86 77.71 ± 4.97 80.20 ± 5.18 77.55 ± 4.29 84.71 ± 4.32 91.82 ± 3.69
2500 75.09 ± 2.11 82.36 ± 3.69 81.97 ± 3.36 85.96 ± 5.79 85.92 ± 4.53 87.85 ± 6.08 97.57 ± 4.11
HIST 25 89.07 ± 4.09 83.60 ± 6.05 87.31 ± 3.25 76.98 ± 6.24 86.51 ± 2.78 82.79 ± 4.26 97.49 ± 1.87 110.62 ± 9.98
50 76.82 ± 2.91 76.13 ± 2.49 80.55 ± 3.72 75.79 ± 6.13 82.68 ± 1.76 82.40 ± 4.00 89.14 ± 5.43
250 79.60 ± 4.57 87.67 ± 2.69 89.50 ± 1.52 83.82 ± 3.11 91.10 ± 1.07 90.02 ± 1.78 98.31 ± 2.39
UDCA 25 94.89 ± 3.47 87.33 ± 5.82 88.31 ± 1.72 89.91 ± 3.04 96.95 ± 3.44 88.43 ± 4.11 99.22 ± 5.23 93.50 ± 7.52
50 82.35 ± 1.91 84.52 ± 2.92 84.54 ± 2.45 92.50 ± 3.34 91.04 ± 1.72 87.98 ± 1.78 84.03 ± 8.11
250 86.26 ± 1.90 85.97 ± 7.65 91.48 ± 1.27 93.68 ± 2.55 97.69 ± 2.33 91.94 ± 2.01 87.18 ± 5.47
CA 25 96.08 ± 5.76 85.92 ± 4.79 87.62 ± 4.87 88.88 ± 4.44 90.85 ± 4.44 88.05 ± 2.63 98.29 ± 5.47 113.92 ± 11.87
50 83.21 ± 4.03 86.54 ± 5.39 88.81 ± 0.78 95.93 ± 6.86 88.62 ± 3.82 87.83 ± 4.07 88.17 ± 5.68
250 84.75 ± 1.06 86.20 ± 4.46 91.26 ± 2.32 91.91 ± 2.36 91.77 ± 2.00 89.53 ± 2.54 95.27 ± 3.48
CDCA 25 95.78 ± 3.83 84.95 ± 3.26 91.83 ± 3.14 91.36 ± 3.29 101.14 ± 4.88 84.76 ± 5.28 75.61 ± 7.91 104.82 ± 10.00
50 87.57 ± 3.66 95.49 ± 5.48 92.60 ± 7.03 104.88 ± 2.04 94.61 ± 5.82 91.13 ± 3.96 70.77 ± 10.45
250 94.50 ± 2.36 91.40 ± 9.45 103.28 ± 2.14 108.03 ± 1.95 107.31 ± 1.46 100.74 ± 5.11 70.42 ± 6.12
DCA 25 97.79 ± 5.06 92.93 ± 1.43 96.25 ± 6.98 96.85 ± 5.17 103.98 ± 4.02 91.67 ± 3.48 85.75 ± 4.44 104.15 ± 10.44
50 90.06 ± 1.98 97.35 ± 3.94 95.48 ± 4.81 107.31 ± 3.66 95.47 ± 5.17 91.59 ± 2.95 74.61 ± 6.28
250 91.50 ± 3.48 97.22 ± 9.01 98.24 ± 1.16 105.35 ± 1.59 106.21 ± 2.83 95.09 ± 4.32 72.67 ± 6.78
LCA 25 98.08 ± 3.26 103.03 ± 3.45 104.43 ± 2.37 107.03 ± 1.78 105.87 ± 3.71 104.61 ± 3.06 87.37 ± 1.46 103.47 ± 10.18
50 93.41 ± 5.14 104.34 ± 1.18 104.08 ± 2.49 107.77 ± 3.97 107.37 ± 3.10 100.29 ± 4.77 76.51 ± 6.28
250 104.65 ± 3.50 101.39 ± 5.81 105.73 ± 7.50 99.73 ± 0.95 109.64 ± 3.94 97.68 ± 4.40 70.83 ± 9.21
  1. *Stabilities and matrix effects were determined in triplicate