Table 1 Method validation data: Retention time, RRF (n = 6), LOD, accuracy, intra-day (RSDR) and inter-day precision (RSDT) (n = 6). Data calculated with the RRF are in bold
Retention time [min] | RRF | LoD [μM] | QC low | QC med | QC high | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
Accuracy (%) | RSDR, (%) | RSDT, (%) | Accuracy (%) | RSDR, (%) | RSDT, (%) | Accuracy (%) | RSDR, (%) | RSDT, (%) | ||||
α-Pinene | 3.8 | 1.8 | 2.7 | 93/93 | 1.2/1.3 | 1.4/1.4 | 95/96 | 2.2/2.2 | 3.4/2.5 | 103/105 | 1.1/1.1 | 2.4/1.7 |
Camphene | 4.5 | 2.2 | 11.0 | 91/91 | 1.7/0.0 | 3.3/2.0 | 95/96 | 2.3/2.7 | 1.8/1.5 | 91/92 | 1.8/1.8 | 3.7/4.4 |
β-Pinene | 5.4 | 1.8 | 5.3 | 93/92 | 1.6/1.6 | 1.8/1.8 | 94/95 | 2.7/2.7 | 4.2/2.9 | 103/105 | 1.4/1.4 | 3.0/2.1 |
3-Carene | 6.5 | 1.6 | 7.3 | 95/93 | 2.0/2.0 | 3.2/2.1 | 95/95 | 3.1/3.1 | 4.3/3.4 | 104/106 | 1.7/1.7 | 3.5/2.4 |
Limonene | 8.4 | 1.8 | 6.6 | 96/94 | 3.3/3.5 | 2.3/2.8 | 97/100 | 3.2/3.2 | 3.1/2.2 | 93/96 | 2.7/2.7 | 6.10/6.1 |
Bornyl acetate | 22.5 | 1.7 | 2.4 | 92/93 | 4.1/4.2 | 4.0/4.6 | 95/96 | 5.6/5.6 | 8.7/6.5 | 105/107 | 3.3/3.3 | 5.6/4.5 |
β-Caryophyllene | 22.8 | 2.3 | 2.8 | 101/100 | 4.9/5.0 | 5.1/5.7 | 103/108 | 6.2/6.2 | 5.6/3.4 | 97/100 | 6.2/6.2 | 7.6/9.1 |
Borneol | 25.5 | 1.7 | 4.3 | 93/96 | 4.3/4.5 | 3.0/3.3 | 97/104 | 4.2/4.2 | 4.0/2.3 | 95/101 | 3.6/3.6 | 5.6/6.8 |