Table 4 Stability data of the five analytes and I.S. in rat plasma under different conditions
Compounds | Nominal concentration (ng/mL) | On bench top at room temperature for 2 h | Freezing at − 70 °C for 30 days | After three freeze/thaw cycles (− 70 °C to 25 °C) | In autosampler rack at 4 °C for 12 h | ||||
|---|---|---|---|---|---|---|---|---|---|
Accuracy (%) | Precision (% CV) | Accuracy (%) | Precision (% CV) | Accuracy (%) | Precision (% CV) | Accuracy (%) | Precision (% CV) | ||
Gastrodin | 20.0 | 94.62 | 1.48 | 95.21 | 10.17 | 106.26 | 6.07 | 102.31 | 1.30 |
2700 | 109.86 | 15.47 | 111.85 | 4.98 | 97.53 | 2.04 | 103.61 | 0.09 | |
Parishin A | 5.0 | 91.97 | 4.53 | 87.79 | 12.01 | 84.98 | 1.52 | 89.52 | 1.13 |
300 | 85.09 | 7.20 | 101.42 | 9.89 | 85.25 | 2.56 | 91.38 | 0.10 | |
Parishin B | 5.0 | 110.51 | 4.21 | 102.78 | 4.85 | 113.57 | 4.50 | 102.78 | 4.85 |
300 | 113.26 | 5.49 | 99.16 | 2.42 | 102.96 | 4.89 | 98.94 | 0.72 | |
Parishin C | 5.0 | 115.42 | 5.36 | 103.81 | 3.68 | 109.40 | 12.45 | 115.42 | 5.36 |
300 | 103.93 | 8.00 | 102.05 | 1.37 | 93.55 | 4.86 | 103.93 | 8.00 | |
Parishin E | 5.0 | 106.28 | 2.50 | 100.69 | 1.58 | 110.3 | 5.97 | 103.56 | 1.81 |
2700 | 108.35 | 2.55 | 104.45 | 5.61 | 86.20 | 6.18 | 103.55 | 9.97 | |
IS | 300 | 100.09 | 7.45 | 99.12 | 0.65 | 109.15 | 12.59 | 98.32 | 3.22 |