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Table 3 Matrix effects (n = 5) and extraction recovery (n = 3) results for analysis of the five analytes and I.S. in rat plasma

From: A simplified LC−MS/MS approach for simultaneous quantification and pharmacokinetics of five compounds in rats following oral administration of Gastrodia elata extract

Compounds

Spiked conc. (ng/mL)

Matrix effect (%)

Extraction recovery (%)

Mean (%)

RSD (%)

Mean (%)

RSD (%)

Gastrodin

20

104.10

8.44

113.95

6.50

2700

89.87

10.72

100.59

10.59

Parishin A

5

84.33

4.56

114.26

3.49

300

92.48

2.52

101.12

0.23

Parishin B

5

94.09

1.77

118.70

1.26

300

106.36

1.71

117.48

1.24

Parishin C

5

103.70

2.91

100.71

2.87

300

105.41

3.57

114.63

2.72

Parishin E

5

115.25

11.67

85.97

13.58

2700

111.13

3.32

108.02

2.84