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Table 2 The recoveries of serum samples spiked with HER-2 and HE4 by dual-label TRFIA

From: Dual-label time-resolved fluoroimmunoassay for simultaneous measurement of human epidermal growth factor receptor 2 and human epididymis protein 4 in serum

SampleHER-2HE4
Spiked/ng ml−1 Observed/ng ml−1, n = 3Recovery (%)CV (%)Spiked/pmol l−1 Observed/pmol l−1, n = 3Recovery (%)CV (%)
Case 109.8  048.1  
2027.68910.150102.3108.45.2
5057.595.43.4100142.1947.1
100106.196.32.6200229.790.83.6
Case 2016.0  031.0  
2034.994.57.35080.298.42.9
5066.7101.41.5100123.992.94.9
100109.693.65.2200237.6103.32.8
Case 3017.5  037.0  
2035.690.52.65087.8101.69.5
5063.491.83.0100127.390.32.6
100115.297.79.1200215.889.43.4
Average  94.55.0  96.64.7