Table 2 The recoveries of serum samples spiked with HER-2 and HE4 by dual-label TRFIA
Sample | HER-2 | HE4 | ||||||
|---|---|---|---|---|---|---|---|---|
Spiked/ng ml−1 | Observed/ng ml−1, n = 3 | Recovery (%) | CV (%) | Spiked/pmol l−1 | Observed/pmol l−1, n = 3 | Recovery (%) | CV (%) | |
Case 1 | 0 | 9.8 | 0 | 48.1 | ||||
20 | 27.6 | 89 | 10.1 | 50 | 102.3 | 108.4 | 5.2 | |
50 | 57.5 | 95.4 | 3.4 | 100 | 142.1 | 94 | 7.1 | |
100 | 106.1 | 96.3 | 2.6 | 200 | 229.7 | 90.8 | 3.6 | |
Case 2 | 0 | 16.0 | 0 | 31.0 | ||||
20 | 34.9 | 94.5 | 7.3 | 50 | 80.2 | 98.4 | 2.9 | |
50 | 66.7 | 101.4 | 1.5 | 100 | 123.9 | 92.9 | 4.9 | |
100 | 109.6 | 93.6 | 5.2 | 200 | 237.6 | 103.3 | 2.8 | |
Case 3 | 0 | 17.5 | 0 | 37.0 | ||||
20 | 35.6 | 90.5 | 2.6 | 50 | 87.8 | 101.6 | 9.5 | |
50 | 63.4 | 91.8 | 3.0 | 100 | 127.3 | 90.3 | 2.6 | |
100 | 115.2 | 97.7 | 9.1 | 200 | 215.8 | 89.4 | 3.4 | |
Average | 94.5 | 5.0 | 96.6 | 4.7 | ||||