Table 1 The cross-reactivity of developed HER-2 and HE4 assay
Interfering substance | Concentration | HE4/pmol l−1 | HER-2/ng ml−1 | ||
|---|---|---|---|---|---|
Observed/pmol l−1 | Cross-reactivity (%) | Observed/ng ml−1 | Cross-reactivity (%) | ||
AFP | 1000 U ml−1 | 2.77 | 0.28 | 2.80 | 0.28 |
CEA | 500 ng ml−1 | 0.29 | 0.06 | 0.00 | 0.00 |
T-PSA | 100 ng ml−1 | 2.75 | 2.75 | 0.00 | 0.00 |
CA242 | 200 U ml−1 | 4.91 | 2.46 | 0.00 | 0.00 |
CA153 | 35 U ml−1 | 0.00 | 0.00 | 0.00 | 0.00 |
CA199 | 300 U ml−1 | 4.03 | 1.34 | 1.94 | 0.65 |
NSE | 350 ng ml−1 | 0.00 | 0.00 | 1.02 | 0.29 |
CA125 | 600 U ml−1 | 0.00 | 0.00 | 0.00 | 0.00 |
HER-2 | 500 ng ml−1 | 0.00 | 0.00 | 500.00 | 100.00 |
HE4 | 2000 pmol l−1 | 1949.00 | 97.45 | 0.30 | 0.02 |