Table 5 HPLC analysis conditions of AI 4~5
From: In vitro and in vivo anti-aging effects of compounds isolated from Artemisia iwayomogi
Detector | UV (340 nm) | ||
Column | AKZO NOBEL. KR100-5C18 (4.6 × 250 mm) | ||
Mobile phase | A—0.1 % acetic acid; B—CH3CN | ||
Time | A (%) | B (%) | |
0 | 70 | 30 | |
30 | 70 | 30 | |
40 | 30 | 70 | |
45 | 70 | 30 | |
60 | 70 | 30 | |
Flow rate | 1.0 mL/min | ||
Injection volume | 20 μL | ||