Removal and tracing of cephalosporins in industrial wastewater by SPE-HPLC: optimization of adsorption kinetics on mesoporous silica nanoparticles

Journal of Analytical Science and Technology

Table 3 Assay parameters and method validation obtained by applying the proposed HPLC-UV method

Parameters	Cefadroxil	Cefprozil	Cefradine
Concentration range (μg/mL)	10–200	10–200	10–200
Slope	0.0257	0.0142	0.013
Intercept	0.0315	0.0031	0.0222
Mean^a ± SD	99.71 ± 1.58	100.47 ± 1.55	99.73 ± 0.70
LOD (μg/mL)	3	4	3
LOQ (μg/mL)	10	10	10
R ²	0.9997	0.9995	0.9999
Accuracy	99.75 ± 1.01	99.90 ± 0.77	100.21 ± 0.246
Inter-day precision^b	0.490	0.535	0.603
Intra-day precision^b	0.620	1.127	0.675

^aAverage of the six concentrations repeated three times
^bRelative standard deviation of the three concentrations (50, 100, and 150 μg mL^− 1) repeated three times (n = 9)