A novel UPLC-PDA isocratic method for the quantification fulvestrant in oil-based pre-filled syringe injection matrix formulations

Journal of Analytical Science and Technology

Table 8 Results of effect of variation in column temperature

S. No.	Name of the parameter	25 °C	30 °C
1	Tailing factor for fulvestrant peak	0.93	0.94
2	% RSD of fulvestrant peak areas for five replicate injections of standard	0.1	0.1
3	USP plate count for fulvestrant peak	4013	3980
4	% Assay	99.3	99.8