Table 1 Fl-FFF operating conditions
FFF channel dimensions (cm) | 28.7 cm long × 1.8 cm wide × 0.021 cm thick |
|---|---|
Sample volume (μL) | 20 |
Delay time (s) | 12 |
Equilibrium time (min) | 2.0 |
Membranes | 1 kDa RC, 10 kDa RC, 10 kDa PES |
Carrier solutions | 0.02% (v/v) FL-70 with 0.02% (w/v) NaN3, ammonium carbonate 0.25 mM |
Channel flow rate (mL min−1) | 1.0 |
Cross flow rate (mL min−1) | 0.8, 0.5a |