Fibrinolytic assays | Characteristics | References |
---|---|---|
Fibrin plate method | • The long incubation period • Uncertainty in the determination of lysis zones | |
Dyed fibrin plate assay | • Lesser incubation period making it rapid • Better accuracy since it is based on colorimetry and not on zone of fibrinolysis • No requirement for elaborate equipment • Uses untreated natural human fibrin. • Requires relatively larger volume of enzyme solution. | (Barta 1966) |
Rapid fibrin plate assay | • Aimed at exploring the plasminogen-enrichment so as to shorten the incubation period • Requires 3 h of incubation as compared to the fibrin plate method that requires 16-20 h of incubation. | (Marsh and Gaffney 1977) |
Solid-phase fibrin plate assay | • 2 h of incubation period required • Multiple samples can be assayed • Highly sensitive | (Millar and Smith 1983) |
Fibrin microplate assay | • Better reliability • Applicable for several analytical tests • One of the possible extension of this method is that the cells that express plasminogen activator or fibrinolytic activity can be applied directly to the gel • Removal of proteinase inhibitors becomes essential for plasma samples | (Fossum and Hoem 1996) |
Plasma streptokinase lysis time | • Streptokinase can be replaced by the test enzyme. • This assay reflects the interplay of all components in the fibrinolytic system with the exception of the activators that are in excess. • A sensitive screening assay for deficient fibrinolytic activity in vivo. | (Gidron et al. 1978) |
Streptokinase-activated lysis time | • Depends on variation in the levels of various components of the fibrinolytic system. | (Gidron et al. 1978) |
Nephelometric method for recording lysis time | • Alternate for Streptokinase activated lysis time | (Gidron et al. 1978) |
Partial clot lysis assay | • Easier method wherein upon clot lysis, the red cells released and the amount of lysis that occurred in a given time can be determined by measuring the number of cells released. | (Howell 1964) |
Hawkey and Stafford assay | • Relatively shorter and simpler assay for quantitative determinations. | (Hawkey and Stafford 1964) |
Esterolytic assays | • Based on hydrolysis of synthetic peptide esters • Very sensitive and suitable for kinetic studies | (Kotb 2013) |