|Fibrin plate method||
• The long incubation period|
• Uncertainty in the determination of lysis zones
|(Fossum and Hoem 1996; Jespersen and Astrup 1983)|
|Dyed fibrin plate assay||
• Lesser incubation period making it rapid|
• Better accuracy since it is based on colorimetry and not on zone of fibrinolysis
• No requirement for elaborate equipment
• Uses untreated natural human fibrin.
• Requires relatively larger volume of enzyme solution.
|Rapid fibrin plate assay||
• Aimed at exploring the plasminogen-enrichment so as to shorten the incubation period|
• Requires 3 h of incubation as compared to the fibrin plate method that requires 16-20 h of incubation.
|(Marsh and Gaffney 1977)|
|Solid-phase fibrin plate assay||
• 2 h of incubation period required|
• Multiple samples can be assayed
• Highly sensitive
|(Millar and Smith 1983)|
|Fibrin microplate assay||
• Better reliability|
• Applicable for several analytical tests
• One of the possible extension of this method is that the cells that express plasminogen activator or fibrinolytic activity can be applied directly to the gel
• Removal of proteinase inhibitors becomes essential for plasma samples
|(Fossum and Hoem 1996)|
|Plasma streptokinase lysis time||
• Streptokinase can be replaced by the test enzyme.|
• This assay reflects the interplay of all components in the fibrinolytic system with the exception of the activators that are in excess.
• A sensitive screening assay for deficient fibrinolytic activity in vivo.
|(Gidron et al. 1978)|
|Streptokinase-activated lysis time||• Depends on variation in the levels of various components of the fibrinolytic system.||(Gidron et al. 1978)|
|Nephelometric method for recording lysis time||• Alternate for Streptokinase activated lysis time||(Gidron et al. 1978)|
|Partial clot lysis assay||• Easier method wherein upon clot lysis, the red cells released and the amount of lysis that occurred in a given time can be determined by measuring the number of cells released.||(Howell 1964)|
|Hawkey and Stafford assay||• Relatively shorter and simpler assay for quantitative determinations.||(Hawkey and Stafford 1964)|
• Based on hydrolysis of synthetic peptide esters|
• Very sensitive and suitable for kinetic studies