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Table 1 List of various fibrinolytic assays

From: Methods available to assess therapeutic potential of fibrinolytic enzymes of microbial origin: a review

Fibrinolytic assays Characteristics References
Fibrin plate method • The long incubation period
• Uncertainty in the determination of lysis zones
(Fossum and Hoem 1996; Jespersen and Astrup 1983)
Dyed fibrin plate assay • Lesser incubation period making it rapid
• Better accuracy since it is based on colorimetry and not on zone of fibrinolysis
• No requirement for elaborate equipment
• Uses untreated natural human fibrin.
• Requires relatively larger volume of enzyme solution.
(Barta 1966)
Rapid fibrin plate assay • Aimed at exploring the plasminogen-enrichment so as to shorten the incubation period
• Requires 3 h of incubation as compared to the fibrin plate method that requires 16-20 h of incubation.
(Marsh and Gaffney 1977)
Solid-phase fibrin plate assay • 2 h of incubation period required
• Multiple samples can be assayed
• Highly sensitive
(Millar and Smith 1983)
Fibrin microplate assay • Better reliability
• Applicable for several analytical tests
• One of the possible extension of this method is that the cells that express plasminogen activator or fibrinolytic activity can be applied directly to the gel
• Removal of proteinase inhibitors becomes essential for plasma samples
(Fossum and Hoem 1996)
Plasma streptokinase lysis time • Streptokinase can be replaced by the test enzyme.
• This assay reflects the interplay of all components in the fibrinolytic system with the exception of the activators that are in excess.
• A sensitive screening assay for deficient fibrinolytic activity in vivo.
(Gidron et al. 1978)
Streptokinase-activated lysis time • Depends on variation in the levels of various components of the fibrinolytic system. (Gidron et al. 1978)
Nephelometric method for recording lysis time • Alternate for Streptokinase activated lysis time (Gidron et al. 1978)
Partial clot lysis assay • Easier method wherein upon clot lysis, the red cells released and the amount of lysis that occurred in a given time can be determined by measuring the number of cells released. (Howell 1964)
Hawkey and Stafford assay • Relatively shorter and simpler assay for quantitative determinations. (Hawkey and Stafford 1964)
Esterolytic assays • Based on hydrolysis of synthetic peptide esters
• Very sensitive and suitable for kinetic studies
(Kotb 2013)