Table 1 Experimental chromatographic conditions for the analysis of ifosfamide, cyclophosphamide, and paclitaxel in workplace wipe samples

Stationary phase

Waters Symmetry® C18, 5 μm, 4.6 × 150 mm column

Mobile phase

Acetonitrile:10 mM phosphate buffer pH 6

0–9.9 min, 25 % A, 75 % B

10–20 min, 60 % A, 40 % B

20.1–25 min, 25 % A, 75 % B

A = 100 % ACN, B = 100 % buffer (KH₂PO₄ 1.1936 g, K₂HPO₄ 0.2143 g, dissolve in 1 L of DI water, adjust at pH 6 with phosphoric acid)

Flow rate

1.0 mL/min

Detection wavelength

195 nm

Injection volume

20 μL