Table 3 Comparison of the present study with the reported GC methods used for determination of purines and pyrimidines

GC-ECD

Pentafluorobenzoyl chloride, pentafluorophenylsulfonyl chloride and heptafluorobutyric anhydride

C, U, and T (standards)

>15.4

Fused silica capillary column, (15 m × 0.25 mm id)

DB-1701

10 nmol–1 μmol mL⁻¹

Nazareth et al. 1984

GC-FID

Bis(trimethylsilyl) trifluoroacetamide

T, U, C, A, and G (yeast and tobacco mosaic virus RNA, salmon sperm, and calf thymus DNA)

24

10 % (weight)

SE-30 on 100/120 mesh “Supelcoport” packed in a 1.0 m × 4.0 mm id glass column

2–50 μmol mL⁻¹

Laking and Gehrke 1972

GC-FID

Trimethylsilyl (TMS)

U, T, A, C, and G (calf thymus DNA)

20

12-m SE-30 fused silica column

0.2–20 μmol mL⁻¹

Gelijkens et al. 1981

GC-NPD

Trimethylsilyl (TMS)

Uracil herbicide residues (bromacil, lenacil, terbacil) (Echinacea angustifolia Moench)

>11

Fused silica capillary column

DB-1

(15 m × 0.32 mm id, 0.25-mm film thickness)

0.1–100 mmol L⁻¹

Tekel et al. 1998

GC-FID

Isobutyl chloroformate (IBCF)

T, U, A, C, and G (human blood, rice, cotton, and jasmine DNA)

10

Column HP-5 (30 m × 0.32 mm id), 0.25-μm layer thickness

0.5–50 μmol mL⁻¹

Present study