Table 1 The backbone chemical shift values of ²H/¹³C/¹⁵N-labeled Ube2g1

G

−2

–

–

–

–

–

A

57

9.503

127.2

49.86

22.54

175.0

H

115

7.069

124.1

54.72

30.58

174.5

S

−1

–

–

–

–

–

H

58

9.315

120.1

52.62

33.37

175.5

T

116

8.170

107.6

59.01

72.11

175.7

M

1

–

–

55.90

31.68

177.0

L

59

8.932

126.4

52.99

45.07

174.9

V

117

7.765

117.9

66.56

30.33

177.7

T

2

8.119

115.8

62.90

69.33

175.2

T

60

8.502

120.1

60.71

69.57

174.5

E

118

8.451

120.8

60.28

28.72

177.2

E

3

8.213

123.2

56.89

29.65

176.8

F

61

9.757

129.0

55.36

39.99

172.5

T

119

7.472

114.9

66.20

68.51

178.1

L

4

8.194

121.9

55.74

41.56

179.0

P

62

–

–

61.28

31.20

177.6

I

120

7.662

120.8

65.11

37.51

177.1

Q

5

8.518

120.3

58.45

27.58

178.6

K

63

8.643

117.6

58.02

30.87

177.6

M

121

8.129

117.8

56.63

29.76

178.6

S

6

8.549

114.3

60.53

62.96

175.9

D

64

8.663

114.8

51.79

37.92

176.2

I

122

8.310

119.6

64.42

36.57

179.5

A

7

7.427

124.7

55.00

17.45

179.5

Y

65

7.540

123.9

57.44

39.46

173.8

S

123

7.513

117.7

62.27

–

177.1

L

8

7.559

118.2

57.68

40.77

180.3

P

66

–

–

63.76

31.51

175.5

V

124

8.253

124.3

65.78

30.33

176.9

L

9

7.987

120.3

57.48

41.19

179.3

L

67

9.231

126.0

57.54

39.98

177.1

I

125

8.402

121.4

65.36

36.25

179.2

L

10

8.319

120.9

58.17

41.01

179.0

R

68

6.707

114.6

51.91

32.00

171.5

S

126

8.008

114.4

61.36

62.69

176.8

R

11

8.246

118.7

59.54

28.98

179.6

P

69

–

–

–

–

–

M

127

7.840

121.8

57.21

30.55

178.5

R

12

7.777

119.9

58.92

29.21

179.1

P

70

–

–

60.94

30.52

175.1

L

128

7.521

120.8

57.13

39.30

176.5

Q

13

8.841

120.0

59.17

27.27

179.4

K

71

7.635

115.5

54.24

33.63

175.3

A

129

7.300

118.2

53.21

19.03

177.8

L

14

8.524

122.1

57.55

40.16

178.5

M

72

8.903

123.9

54.10

35.54

172.5

D

130

7.988

117.4

51.59

41.66

171.1

A

15

7.681

120.7

54.63

17.17

181.5

K

73

8.496

128.2

53.74

36.00

176.7

P

131

–

–

62.34

30.49

174.7

E

16

8.288

118.7

58.83

28.60

179.2

F

74

9.856

126.5

60.52

38.48

175.7

N

132

8.756

120.0

52.39

39.24

176.4

L

17

7.725

120.3

56.89

40.93

178.6

I

75

9.188

124.5

60.12

36.30

177.5

G

133

8.543

112.1

45.15

–

173.9

N

18

7.801

115.4

54.72

38.64

176.6

T

76

7.493

119.1

62.88

70.02

173.7

D

134

8.088

120.2

55.10

40.58

176.0

K

19

7.638

119.6

57.57

32.35

176.1

E

77

8.430

127.3

57.58

28.92

175.2

S

135

7.943

114.2

55.17

62.81

172.3

N

20

7.778

117.3

50.71

39.33

170.8

I

78

8.579

124.0

60.03

40.20

171.0

P

136

–

–

62.62

31.67

175.8

P

21

–

–

63.18

31.45

177.3

W

79

6.210

128.0

54.53

30.44

174.3

A

137

8.065

125.8

52.67

19.43

177.5

V

22

8.366

122.4

60.77

32.50

175.9

H

80

9.352

128.3

55.77

33.58

172.9

N

138

7.734

115.7

50.57

36.89

176.1

E

23

8.346

124.6

57.57

28.90

177.4

P

81

–

–

65.07

32.49

175.9

V

139

8.585

121.6

65.06

30.75

178.0

G

24

8.346

110.0

45.38

–

173.1

N

82

11.510

117.2

53.59

39.85

172.0

D

140

7.742

122.3

57.12

40.27

177.8

F

25

7.442

114.9

55.99

41.20

173.6

V

83

7.452

120.5

60.35

32.74

175.4

A

141

6.837

122.3

53.66

15.46

179.3

S

26

8.546

114.6

56.94

65.37

172.7

D

84

8.792

126.1

53.05

41.44

178.4

A

142

8.023

118.8

55.18

17.99

178.6

A

27

8.840

124.6

50.62

23.41

175.7

K

85

8.790

121.9

58.95

31.35

177.3

K

143

7.883

120.0

59.68

31.50

178.5

G

28

8.307

107.1

44.05

–

171.0

N

86

8.780

117.5

52.81

38.07

176.0

E

144

7.896

118.7

58.69

30.26

179.1

L

29

8.361

118.0

53.97

39.98

178.3

G

87

8.445

109.2

44.75

–

174.7

W

145

8.504

120.2

60.44

–

176.9

I

30

7.412

124.6

64.49

38.13

175.7

D

88

8.395

123.6

56.10

40.05

176.1

R

146

7.757

115.7

58.51

30.48

179.1

D

31

9.493

121.9

52.30

43.62

176.4

V

89

8.188

123.9

63.17

31.62

175.7

E

147

8.419

116.0

57.12

30.45

177.2

D

32

8.513

124.4

56.42

40.27

176.1

C

90

8.551

130.3

57.66

27.00

173.3

D

148

8.827

122.5

53.07

40.86

177.1

N

33

8.458

114.4

53.05

38.80

174.7

I

91

6.766

115.6

58.81

40.26

176.6

R

149

8.222

125.0

59.80

29.47

177.3

D

34

8.294

121.8

52.43

41.39

175.3

S

92

8.585

120.6

62.06

–

–

N

150

8.177

111.2

52.87

38.40

174.6

L

35

8.587

125.1

55.39

41.48

175.7

I

93

7.862

119.4

63.14

37.07

172.7

G

151

7.442

110.9

44.89

–

174.2

Y

36

8.271

111.8

59.78

36.54

175.7

L

94

6.774

113.0

52.64

39.65

176.8

E

152

9.192

131.1

58.63

29.53

177.5

R

37

6.793

116.6

55.31

31.12

175.1

H

95

7.955

119.6

53.56

29.57

174.7

F

153

7.581

116.1

62.82

37.62

176.4

W

38

9.575

127.3

55.05

31.35

175.6

E

96

8.948

123.9

54.88

28.75

175.1

K

154

6.681

118.0

59.38

31.45

177.8

E

39

9.297

122.3

54.72

–

175.0

P

97

–

–

63.85

31.29

177.4

R

155

8.108

118.8

59.59

29.62

180.0

V

40

9.435

123.9

58.59

34.35

173.4

G

98

8.505

108.7

44.63

–

174.2

K

156

8.094

120.6

60.10

32.41

180.8

L

41

8.659

126.8

53.50

42.95

175.7

E

99

7.919

120.9

56.34

29.54

176.6

V

157

8.516

122.6

67.29

30.39

178.2

I

42

9.200

125.9

59.51

39.97

174.6

D

100

8.396

123.2

54.14

40.69

176.7

A

158

8.867

123.2

55.07

16.80

180.9

I

43

8.245

127.9

57.56

36.11

177.8

K

101

8.182

121.4

56.46

31.55

176.2

R

159

7.515

118.1

59.08

28.61

178.5

G

44

9.369

114.8

43.58

–

172.7

Y

102

7.945

119.1

57.22

37.34

176.5

C

160

7.666

118.9

61.60

25.70

178.3

P

45

–

–

–

–

–

G

103

8.034

109.0

45.35

–

173.8

V

161

8.912

125.7

66.68

30.83

179.3

P

46

–

–

62.52

31.29

176.4

Y

104

7.686

118.8

57.26

37.64

175.9

R

162

8.310

122.3

58.86

28.59

179.4

D

47

8.986

115.6

55.80

38.92

175.3

E

105

8.147

122.8

56.03

29.92

176.1

K

163

8.266

119.1

58.63

31.02

179.4

T

48

7.466

106.1

60.16

73.96

177.0

K

106

8.470

122.8

53.97

31.24

175.6

S

164

7.830

116.0

60.90

61.81

175.0

L

49

9.598

121.7

56.55

40.85

176.4

P

107

–

–

64.70

30.89

178.1

Q

165

7.250

121.0

56.34

27.95

177.2

Y

50

7.351

113.7

57.88

38.34

174.0

E

108

9.028

115.9

57.59

27.85

176.7

E

166

7.697

120.0

57.06

29.34

177.4

E

51

7.091

122.3

57.58

29.31

176.7

E

109

7.878

119.7

55.72

29.27

175.4

T

167

7.921

113.3

61.93

69.40

174.5

G

52

8.599

114.7

44.49

–

175.2

R

110

7.356

119.5

54.13

31.18

174.6

A

168

7.847

125.8

52.53

18.37

177.4

G

53

8.524

108.0

45.17

–

171.2

W

111

8.424

120.1

59.72

29.52

175.7

F

169

8.011

118.8

57.02

38.88

174.9

V

54

7.437

124.1

61.03

31.50

174.4

L

112

5.885

126.6

50.43

45.26

–

E

170

7.671

126.9

57.52

30.31

180.9

F

55

9.057

124.4

56.11

40.79

174.1

P

113

–

–

64.22

–

175.7

K

56

9.290

123.9

55.09

33.10

176.1

I

114

5.597

107.4

60.18

37.30

176.6