Table 2 Role of pH in plasma on inter-conversion (n = 4)
Analysis of LS spiked sample (10.0 ng/mL) | ||||||
|---|---|---|---|---|---|---|
Duration | Normal plasma | Acidified plasma (pH 6.0) | Acidified plasma (pH 4.0) | |||
A1 | A2 | A1 | A2 | A1 | A2 | |
0.0 h | 588,767 | 14,529 | 722,551 | 2157 | 621,396 | 2548 |
2.0 h | 565,406 | 21,013 | 718,763 | 2478 | 632,705 | 2989 |
4.0 h | 533,753 | 25,785 | 729,776 | 2054 | 634,905 | 3054 |
6.0 h | 482,788 | 27,854 | 716,005 | 2248 | 604,583 | 2689 |
Analysis of LHA spiked sample (10.0 ng/mL) | ||||||
Duration | Normal plasma | Acidified plasma (pH 6.0) | Acidified plasma (pH 4.0) | |||
A1 | A2 | A1 | A2 | A1 | A2 | |
0.0 h | 9642 | 37,598 | 2034 | 46,998 | 6556 | 43,560 |
2.0 h | 13,925 | 33,423 | 2496 | 42,578 | 10,026 | 40,527 |
4.0 h | 16,780 | 33,168 | 2738 | 42,895 | 11,947 | 38,952 |
6.0 h | 19,962 | 31,313 | 2568 | 44,224 | 15,890 | 34,744 |