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Table 1 Δ T m of protein for ten compounds (C1 to C10)

From: Acquisition of data at multiple gains within a single thermal melt experiment using the Rotor-Gene Q instrument

Concentration of compounds C1 to C10 (mM) The values of Δ T m at different gains used in the same experimental run (°C) Average Δ T m (°C)
Gain −2 Gain 0 Gain 2 Gain 4 Gain 6
0.5   0.83 0.93 0.93 0.63 - 0.82 ± 0.14
2 C1 1.13 1.63 1.43 - - 1.39 ± 0.25
5   1.63 2.13   - - 1.87 ± 0.35
0.5   0.83 1.33 1.13 0.93 - 1.05 ± 0.22
2 C2 1.13 1.63 1.63 1.33 - 1.42 ± 0.24
5   1.13 1.63 1.83   - 1.52 ± 0.36
0.5   0.33 0.83 0.63 0.63 - 0.60 ± 0.21
2 C3 −0.08 0.33 0.13 0.13 - 0.12 ± 0.16
5   0.43 0.33 0.43 0.13 - 0.32 ± 0.14
0.5   0.63 1.13 1.33 0.93 - 1.00 ± 0.30
2 C4 2.93 3.63 3.43 3.13 - 3.27 ± 0.31
5   −0.08 0.43 0.63   - 0.32 ± 0.36
0.5   0.43 0.63 0.63 0.43 - 0.52 ± 0.12
2 C5 −0.18 0.13 0.13 −0.18 - −0.03 ± 0.17
5   0.33 0.63 0.83 - - 0.59 ± 0.25
0.5   0.63 0.83 0.63 0.33 - 0.60 ± 0.21
2 C6 0.63 0.83 0.63 0.33 0.33 0.55 ± 0.22
5   0.33 0.33 0.13 −0.08 −0.18 0.11 ± 0.23
0.5   0.43 1.43 0.93 - - 0.93 ± 0.05
2 C7 −0.18 0.13 - - - −0.03 ± 0.21
5   - - - - - -
0.5   1.13 1.43 1.13 0.83 0.63 1.03 ± 0.31
2 C8 1.13 1.63 1.63 1.33 - 1.43 ± 0.24
5   2.43 2.83 3.13   - 2.79 ± 0.35
0.5   - 0.33 0.13 −0.38 −0.38 −0.08 ± 0.36
2 C9 - 1.33 1.13 0.93 0.83 1.05 ± 0.22
5   2.63 3.63 3.63 3.13 3.13 3.23 ± 0.42
0.5   0.43 0.83 0.63 0.43 - 0.58 ± 0.19
2 C10 1.13 1.63 1.43 1.13 - 1.33 ± 0.24
5   1.83 2.33 2.33 1.93 - 2.10 ± 0.26
  1. Hyphen indicates samples that did not show fluorescence melting signal above threshold noise level or resulted in signal saturation (data loss) due to high fluorescence intensity. The ΔT m of protein was measured for the ten compounds C1 to C10 at three concentrations (0.5, 2, and 5 mM) at five levels of gain set within a single experimental run. T m of the protein (T m ref) = 58.4 ± 0.2°C.