Figure 4
Titration of compound 8 with the target protein. (A) Fluorescence melting curves showing change in fluorescence intensity with increasing temperature for different concentrations of C8 shown in the figure. (B) First derivative of melting curves (dF/dT) is plotted and the temperature at maximum dF/dT is the protein melting temperature (T m). The data for gain level of 5 are shown.