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Table 2 Optimization of the treatment method of Zhuyeqing Liquor (μg/mL)

From: Simultaneous quantification of major bioactive constituents from Zhuyeqing Liquor by HPLC-PDA

Compounda

Treatment method

Method 1

Method 2

Method 3

Method 4

Method 5

M1

ND

5.2494

3.1331

9.5943

16.4270

M2

0.0888

0.5371

0.5078

0.5818

0.5942

M3

0.0922

0.0906

0.0388

0.0939

0.1063

M4

0.0263

0.4980

0.5098

0.5069

0.5153

M5

ND

44.0545

101.6907

100.6372

101.7888

M6

0.1479

0.1880

0.1902

0.1927

0.1936

M7

45.2421

563.2436

570.3556

566.0022

574.2514

M8

20.8481

24.6279

25.6049

24.5513

25.7319

M9

0.1931

0.1782

0.1906

0.1918

0.1968

M10

0.0324

0.0526

0.0600

0.0705

0.0736

M11

0.2009

0.4601

0.4871

0.4915

0.4954

M12

0.7071

1.0544

1.0683

1.0699

1.0877

M13

1.1371

2.5392

2.8461

2.8029

2.9909

M14

ND

3.4939

4.0839

4.0563

4.3756

M15

ND

0.0641

0.0678

0.0688

0.0689

M16

0.5189

0.6113

0.5842

0.3282

0.6623

M17

2.9599

2.9776

2.1520

1.6446

2.9957

M18

0.4448

0.5307

0.4278

0.4673

0.5413

Sumb

72.6396

650.4512

713.9987

713.3521

733.0967

  1. Sample in optimization of the treatment method was 45° Zhuyeqing Liquor (20130207). Method 1, acetoacetate extract; method 2, n-butanol extract; method 3, 70% ethanol treatment; method 4, SPE column eluted with methanol; method 5, HPD100 column eluted with ethanol. a‘ND’ in the ‘Compound’ column expressed under LOQ. bTotal content of the 18 investigated compounds.