Table 1 Chromatographic conditions
Composition | Value |
|---|---|
Column | C18 reverse phase column (250???4.6?mm, particle size 5??m; Merck, Darmstadt, Germany) |
Flow rate | 1?mL?min-1 |
Retention time | 7.968???0.042?min |
Detector | PDA detector (Waters 2998) |
Detection wavelength | 280?nm |
Injection volume | 20??L |
Temperature | 25?C |
Elution type | Isocratic |
Run time | 20?min |