Figure 3

Effect of 10 ?M Y-27632 on the maintenance of endothelium integrity in organ-cultured mesenteric arteries. (A) Representative images of the endothelial layer that were immunostained with anti-PECAM (CD31) antibody in the longitudinal sections of freshly isolated and organ-cultured mesenteric arteries. To verify the morphological integrity of the endothelial cell layer, elastin autofluorescence (Auto Fl; green) and cell nucleus (DAPI; blue) were also captured with PECAM (red). (B) Representative immunoblot images of PECAM and calponin (as a loading marker) and a quantitative summary (lower) for the relative PECAM expression (n = 3). The asterisk represents a significant (P < 0.05) difference from that of the fresh mesenteric artery. The number sign represents a significant (P < 0.05) difference from that of the FBS-supplemented organ-cultured mesenteric artery in the absence of Y-27632.