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Journal of Analytical Science and Technology
Figure 1 | Journal of Analytical Science and Technology

Figure 1

From: Critical importance of the correction of contrast transfer function for transmission electron microscopy-mediated structural biology

Figure 1

Overview of image processing of a 2D crystal. Raw electron micrograph is Fourier transformed, from which unit cell parameter for the crystal lattice is determined. Fourier transform (FFT) is also used to determine image defocus and CTF fitting. Diffraction spots from FFT are masked in order to eliminate background noise. A filtered image that results from inverse Fourier transformation of masked FFT serves as a reference for cross-correlation-based image unbending. FFT from unbent image is CTF-corrected, and final projection image is generated by inverse Fourier transform.

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