Table 1 Intra- and inter-day precision values for PC spiked in human plasma for the three extraction methods using positive-ion UPLC-MS
| Â | Â | Matyash | SAP | mSAP | |||
|---|---|---|---|---|---|---|---|
Measured peak area | RSD (%) | Measured peak area | RSD (%) | Measured peak area | RSD (%) | ||
Intra-day | Day 1 | 6382.97 ± 259.99 | 4.07 | 5143.58 ± 878.99 | 17.09 | 6167.90 ± 287.13 | 4.66 |
|  | Day 2 | 5504.57 ± 729.44 | 13.25 | 5235.33 ± 402.89 | 7.70 | 5888.58 ± 321.31 | 5.46 |
|  | Day 3 | 5355.43 ± 556.56 | 10.39 | 5747.02 ± 985.05 | 17.14 | 6269.25 ± 265.23 | 4.24 |
|  | Day 4 | 5995.99 ± 417.61 | 6.96 | 5839.61 ± 1272.82 | 21.80 | 6337.83 ± 102.23 | 1.61 |
|  | Day 5 | 5625.93 ± 706.49 | 12.56 | 5924.22 ± 900.96 | 15.21 | 5845.24 ± 184.58 | 3.16 |
|  | Ave | – | 9.45 | – | 15.79 | – | 3.82 |
Inter-day | 5772.98 ± 638.39 | 11.06 | 5577.95 ± 912.03 | 16.35 | 6101.76 ± 302.76 | 4.96 | |