Table 1 Gradient elution condition for liraglutide LC–MS/MS
Time (min) | Mobile phase A | Mobile phase B | Flow rate (mL/min) |
|---|---|---|---|
0.3% Formic acid in distilled water (%) | 0.3% Formic acid in acetonitrile (%) | ||
0.00 | 65 | 35 | 0.3 |
5.00 | 20 | 80 | 0.3 |
5.01 | 0 | 100 | 0.3 |
9.00 | 0 | 100 | 0.3 |
9.01 | 65 | 35 | 0.4 |
13.50 | 65 | 35 | 0.4 |
13.51 | 65 | 35 | 0.3 |