Table 4 Uncertainty sources in the ID–LC/MS method for the determination of biotin in multivitamins
From: Development of a certified reference material for the analysis of vitamins in multivitamin tablets
Group | Uncertainty components | Sources (evaluation methods) | Typical valuea (relative %) |
|---|---|---|---|
Ib | Standard solution | Purity of the reference material (from the KRISS purity analysis) | 0.1 |
Gravimetric preparation (from cross-check of independent sets of calibration solutions) | 0.2 | ||
Isotope ratio standard | Gravimetric mixing (from cross-check of multiple isotope ratio standards from each individual standard solution) | 0.2 | |
Peak area ratio of biotin and 2H2-biotin from LC/MS measurements of isotope ratio standard | Repeatability of multiple measurements | 0.3 | |
IIc | Mass of sample taken for analysis | Readability and linearity of the balance used (from the certificate of the balance) | < 0.01 |
Mass of 2H2-biotin solution spiked into sample taken for analysis | Readability and linearity of the balance used (from the certificate of the balance) | < 0.01 | |
Peak area ratio of biotin and 2H2-biotin from LC/MS measurements of sample extract | Repeatability of multiple measurements | 0.1–0.3 |