A short novel antimicrobial peptide BP100-W with antimicrobial, antibiofilm and anti-inflammatory activities designed by replacement with tryptophan

Journal of Analytical Science and Technology

Table 1 Amino acid sequences and physicochemical properties of BP100 and BP100-W

Peptides	Amino acid sequence	Molecular mass (Da)		Net charge	t_R^b	μH^c
Peptides	Amino acid sequence	Theoretical	Measured^a	Net charge	t_R^b	μH^c
BP100	KKLFKKILKYL-NH₂	1421.9	1420.74	+ 5	17.278	0.847
BP100-W	KKWFKKILKYL-NH₂	1494.9	1493.79	+ 5	18.548	0.873

Substituted amino acids are shown in bold and underlined
^aMolecular mass was determined using matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF)-mass spectrometry (MS)
^bRetention time (t_R) was measured using a C-18 reverse phase analytical high-performance liquid chromatography (HPLC) column (5 mm; 4.6 mm × 250 mm; Vydac). The peptides were eluted for 60 min using a linear gradient of 0 − 90% (v/v) acetonitrile in water containing 0.05% (v/v) trifluoroacetic acid (TFA)
^cHydrophobicity moment (μH) was calculated online at: http://heliquest.ipmc.cnrs.fr/cgi-bin/ComputParams.py