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Journal of Analytical Science and Technology
Fig. 6 | Journal of Analytical Science and Technology

Fig. 6

From: A short novel antimicrobial peptide BP100-W with antimicrobial, antibiofilm and anti-inflammatory activities designed by replacement with tryptophan

Fig. 6

a Effects of BP100 and BP100-W on nitric oxide (NO) production in lipopolysaccharide (LPS)-stimulated RAW264.7 cells. b Effects of BP100 and BP100-W on tumor necrosis factor (TNF)-α release from LPS-stimulated RAW264.7 cells. Concentration of peptides used for this experiment is 8 μM. All data represent at least three independent experiments and are expressed as the mean ± standard error of the mean (SEM). Data were analyzed using one-way analysis of variance (ANOVA) with Bonferroni’s post-test. Asterisks indicate the statistically significant differences (*P < 0.001 for each agonist). c Effects of BP100 and BP100-W on the mRNA levels of inducible nitric oxide synthase (iNOS) and TNF-α in LPS-stimulated RAW264.7 cells. RAW264.7 cells (5 × 105 cells/well) were incubated with the peptides in the presence of LPS (20 ng/mL) for 3 h (for TNF-α) or 6 h (for iNOS). Total RNA was isolated and analyzed to determine the mRNA levels of iNOS and TNF-α using reverse transcription-polymerase chain reaction (RT-PCR). Concentration of peptides used for this experiment is 8 μM

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