Table 2 The stability of mefatinib in human plasma under a variety of storage and process conditions (n = 6)
Concentration (ng mL−1) | Short-term stability (room temp/6 h) | Long-term stability (− 70 °C/386 d) | Autosampler stability (4 °C/24 h) | Freeze–Thaw stability (4 cycles) | Dilution stability (dilution factor = 3) | |||||
|---|---|---|---|---|---|---|---|---|---|---|
Mean ± SD (ng mL−1) | CV (%) | Mean ± SD (ng mL−1) | CV (%) | Mean ± SD (ng mL−1) | CV (%) | Mean ± SD (ng mL−1) | CV (%) | Mean ± SD (ng mL−1) | CV (%) | |
1.00 (LQC) | 0.988 ± 0.0340 | 3.46 | 0.985 ± 0.0960 | 9.72 | 1.01 ± 0.0451 | 4.46 | 0.997 ± 0.00234 | 0.23 | NA | NA |
160 (HQC) | 155 ± 0.624 | 1.25 | 161 ± 5.40 | 3.36 | 156 ± 0.416 | 0.27 | 142 ± 2.76 | 1.94 | NA | NA |
300 (Dil-QC) | NA | NA | NA | NA | NA | NA | NA | NA | 296 ± 3.19 | 1.08 |