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Journal of Analytical Science and Technology
Fig. 3 | Journal of Analytical Science and Technology

Fig. 3

From: Dehydrocostus lactone ameliorates lipid accumulation, insulin resistance, and endoplasmic reticulum stress in palmitate-treated hepatocytes

Fig. 3

DHE decreases ROS levels and protein levels of ER stress markers in PA-treated HepG2 cells. a HepG2 cells were treated with 0.25 mM PA or with 0.25 mM PA and 10 μM DHE for 24 h, and ROS levels were assessed (Scale bar = 50 μm). b ROS levels were evaluated in the treated cells using SpectroMax M4. c The treated HepG2 cells were assessed for the protein expression of ER stress markers (PERK, IRE1α, ATF6, Bip, p-JNK(T1183/Y185), and p-eIF2α (S51)) using western blotting. d Relative protein expression levels of ER stress markers were assessed using the Image J program. Values were normalized to β-actin, JNK, or eIF2α. All data are indicated as mean ± SD of at least three independent experiments. ***p < 0.001, **p < 0.01, *p < 0.05. n = 3–5. CTL; control

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