Storage condition | Nominal concentration (ng/mL) | ||
---|---|---|---|
LQC | MQC | HQC | |
3 | 150 | 1600 | |
(A) Stability in mouse plasma | |||
Bench top (room temperature for 6 h) | 104 ± 4 | 102 ± 0 | 99.1 ± 1.1 |
Long term (− 20 °C for 1 month) | 96.0 ± 1.6 | 101 ± 2 | 100 ± 2 |
Freeze–thaw (3 cycles) | 104 ± 1 | 110 ± 1 | 99.5 ± 4.1 |
Autosampler (10 °C for 10 h) | 99.9 ± 3.3 | 91.0 ± 4.9 | 93.7 ± 7.5 |
(B) Stability in human plasma | |||
Bench top (room temperature for 6 h) | 102 ± 1 | 103 ± 1 | 102 ± 1 |
Long term (− 20 °C for 1 month) | 104 ± 1 | 100 ± 3 | 100 ± 1 |
Freeze–thaw (3 cycles) | 105 ± 1 | 104 ± 1 | 97.9 ± 1.0 |
Autosampler (10 °C for 10 h) | 108 ± 1 | 106 ± 1 | 102 ± 1 |