Table 1 Intra-/inter-day accuracy and precision of alpelisib in mouse and human plasma
Nominal concentration (ng/mL) | ||||
|---|---|---|---|---|
LLOQ | LQC | MQC | HQC | |
1 | 3 | 150 | 1600 | |
(A) Mouse plasma | ||||
Intra-day (n = 6) | ||||
Measured concentration (ng/mL; mean ± SD) | 1.00 ± 0.03 | 3.05 ± 0.27 | 149 ± 6 | 1530 ± 70 |
Accuracy (RE, %) | 0.000 | 1.61 | − 0.667 | − 4.17 |
Precision (RSD, %) | 3.23 | 8.85 | 4.29 | 4.42 |
Inter-day (n = 18) | ||||
Measured concentration (ng/mL; mean ± SD) | 0.998 ± 0.030 | 2.97 ± 0.19 | 145 ± 9 | 1570 ± 60 |
Accuracy (RE, %) | − 0.189 | − 1.11 | − 3.26 | − 1.98 |
Precision (RSD, %) | 3.02 | 6.37 | 6.15 | 3.80 |
(B) Human plasma | ||||
Intra-day (n = 6) | ||||
Measured concentration (ng/mL; mean ± SD) | 0.998 ± 0.010 | 3.08 ± 0.09 | 154 ± 1 | 1550 ± 10 |
Accuracy (RE, %) | − 1.13 | 2.56 | 2.33 | − 3.13 |
Precision (RSD, %) | 0.961 | 2.97 | 0.798 | 0.816 |
Inter-day (n = 18) | ||||
Measured concentration (ng/mL; mean ± SD) | 0.977 ± 0.019 | 3.03 ± 0.12 | 153 ± 6 | 1610 ± 70 |
Accuracy (RE, %) | − 2.29 | 0.963 | 2.30 | 0.451 |
Precision (RSD, %) | 1.98 | 3.83 | 3.76 | 4.09 |