Table 3 Precision, accuracy, recovery, and matrix effects of aflatoxins in blood
Analyte | Spiked concentration (ng/mL) | Intraday(n = 6) | Inter-day (6 × 4 days) | Recovery (mean ± SD, %) | Matrix effects (mean ± SD, %) | ||
|---|---|---|---|---|---|---|---|
accuracy (%) | Precision (%) | accuracy (%) | Precision (%) | ||||
AFB1 | 0.05 | 95 | 4 | 99 | 3 | 78 ± 3 | 67 ± 1 |
0.5 | 102 | 1 | 100 | 5 | 85 ± 4 | 65 ± 2 | |
2 | 100 | 2 | 99 | 5 | 79 ± 6 | 66 ± 1 | |
16 | 97 | 1 | 99 | 3 | 70 ± 1 | 63 ± 1 | |
AFB2 | 0.05 | 104 | 4 | 100 | 5 | 80 ± 1 | 82 ± 1 |
0.5 | 106 | 4 | 99 | 6 | 86 ± 3 | 68 ± 2 | |
2 | 93 | 4 | 98 | 7 | 80 ± 5 | 67 ± 1 | |
16 | 106 | 2 | 99 | 4 | 72 ± 2 | 63 ± 1 | |
AFG1 | 0.05 | 105 | 1 | 100 | 4 | 75 ± 1 | 80 ± 1 |
0.5 | 105 | 4 | 99 | 4 | 93 ± 4 | 76 ± 3 | |
2 | 95 | 4 | 99 | 6 | 82 ± 5 | 79 ± 0.4 | |
16 | 106 | 3 | 100 | 4 | 70 ± 2 | 74 ± 1 | |
AFM1 | 0.05 | 104 | 5 | 102 | 4 | 75 ± 3 | 107 ± 2 |
0.5 | 98 | 2 | 98 | 5 | 88 ± 2 | 99 ± 1 | |
2 | 92 | 1 | 94 | 5 | 89 ± 6 | 103 ± 2 | |
16 | 98 | 1 | 98 | 2 | 73 ± 3 | 96 ± 2 | |
AFG2 | 0.2 | 105 | 5 | 100 | 4 | 75 ± 2 | 72 ± 2 |
0.5 | 97 | 6 | 98 | 5 | 95 ± 2 | 70 ± 2 | |
2 | 111 | 3 | 99 | 8 | 81 ± 2 | 74 ± 1 | |
16 | 90 | 4 | 98 | 6 | 68 ± 3 | 67 ± 1 | |
AFM2 | 0.2 | 111 | 6 | 101 | 4 | 73 ± 1 | 100 ± 2 |
0.5 | 96 | 8 | 101 | 4 | 96 ± 5 | 105 ± 2 | |
2 | 103 | 5 | 95 | 5 | 89 ± 1 | 100 ± 0.5 | |
16 | 96 | 2 | 99 | 3 | 73 ± 3 | 102 ± 3 | |