Table 1 Box-Behnken experimental design and response values for normalized output signal change
Run | Incubation time of the studied sample with CMC (X1, min) | GOD concentration (X2, U/ml) | Assessment time (reaction depth) (X3, sec) | Normalized sensor signal change ΔI | |||
|---|---|---|---|---|---|---|---|
Real value | Coded level | Real value | Coded level | Real value | Coded level | ||
1 | 40 | 0 | 12 | 1 | 300 | −1 | 0.56 |
2 | 40 | 0 | 12 | 1 | 600 | 1 | 0.64 |
3 | 40 | 0 | 4 | −1 | 600 | 1 | 0.47 |
4 | 40 | 0 | 8 | 0 | 450 | 0 | 0.46 |
5 | 10 | −1 | 8 | 0 | 300 | −1 | 0.34 |
6 | 10 | −1 | 4 | −1 | 450 | 0 | 0.27 |
7 | 70 | 1 | 12 | 1 | 450 | 0 | 0.73 |
8 | 70 | 1 | 8 | 0 | 600 | 1 | 0.71 |
9 | 40 | 0 | 8 | 0 | 450 | 0 | 0.39 |
10 | 70 | 1 | 8 | 0 | 300 | −1 | 0.60 |
11 | 10 | −1 | 8 | 0 | 600 | 1 | 0.26 |
12 | 40 | 0 | 8 | 0 | 450 | 0 | 0.48 |
13 | 40 | 0 | 4 | −1 | 300 | −1 | 0.48 |
14 | 40 | 0 | 8 | 0 | 450 | 0 | 0.51 |
15 | 40 | 0 | 8 | 0 | 450 | 0 | 0.46 |
16 | 70 | 1 | 4 | −1 | 450 | 0 | 0.70 |
17 | 10 | −1 | 12 | 1 | 450 | 0 | 0.33 |