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Journal of Analytical Science and Technology
Fig. 3 | Journal of Analytical Science and Technology

Fig. 3

From: Shotgun proteomics of extracellular matrix in late senescent human dermal fibroblasts reveals a down-regulated fibronectin-centered network

Fig. 3

Immunoblot analysis of ECM proteins. A Images of expressed ECM proteins in passage #15 and #40 HDFs. Equal amount of ECM proteins (45 μg) from sub-confluent young and late senescent fibroblasts were used for immunoblot analysis with antibodies against FN1, VTN, HTRA1, CAV1, and APOB. B Quantification of protein expression levels. Relative expression levels of ECM proteins were quantified with β-actin as a house-keeping protein. Quantification was performed using Image J program. ***Statistical significance P < 0.001

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